Hemoglobin Electrophoresis Evaluation

Clinical Significance

Diagnosis of thalassemias and hemoglobin variants
Evaluation of unexplained microcytosis

Specimen Requirements

10.0 mL Whole Blood in (2) two Lavender Top Tubes - EDTA

Transport And Storage

7 days Refrigerated

Collection Instructions

Patient age required on order with all other requirements

Recent Transfusion information should be sent with specimen

Send in Lavendar tubes / Do not aliquot

Minimum volume notes
3mL if multiplex ligation-dependent probe amplification is desired

Minimum Volume

1.0 - 3.0 mL Whole Blood

Neonatal Volume

Reference Range:

Hemoglobin A:
1-30 days: 5.9-77.2%
1-2 months: 7.9-92.4%
3-5 months: 54.7-97.1%
6-8 months: 80.0-98.0%
9-12 months: 86.2-98.0%
13-17 months: 88.8-98.0%
18-23 months: 90.4-98.0%
> or =24 months: 95.8-98.0%

 Hemoglobin A2:
1-30 days: 0.0-2.1%
1-2 months: 0.0-2.6%
3-5 months: 1.3-3.1%
> or =6 months: 2.0-3.3%
 
Hemoglobin F
1-30 days: 22.8-92.0%
1-2 months: 7.6-89.8%
3-5 months: 1.6-42.2%
6-8 months: 0.0-16.7%
9-12 months: 0.0-10.5%
13-17 months: 0.0-7.9%
18-23 months: 0.0-6.3%
> or =24 months: 0.0-0.9%

Varient: No abnormal variants
Varient 2:No abnormal variants
Varient 3:No abnormal variants

Methodology:

• Multiple - Based on Individual Components

Clinical Significance

Diagnosis of thalassemias and hemoglobin variants
Evaluation of unexplained microcytosis

CDM

00913333

CPT Coding

83020, 83021 (Reflex 82664, 83068, 83789, 88184)

Medical Necessity May Apply