Last Modified: 4/13/2021 5:34:37 AM

Medical Necessity Documentation:  
Client Notes:  
Patient Preparation:  
Specimen Requirements:

3.0 mL Whole Blood in a Green Top Tube - Na Heparin 
3.0 mL Whole Blood in a Lavender Top Tube - EDTA

Collection Instructions:  
Transport & Storage: Temperature/Stability: 48 hours Ambient
Rejection Criteria: Specimens with Lithium Heparin; Specimen suitability will be determined by the flow cytometry department only
Reference Range:
Pediatric (less than 3 years)
CD3 (Mature T-cells): 55% - 82% / 2324 - 3295 cells/uL
CD4 (Helper T-cells): 38% - 46% / 1538 - 2213 cells/uL
CD8 (Suppressor T-Cells): 8% - 31% / 351 - 2479 cells/uL
CD4/CD8 Ratio: 1.2 - 6.2

CD3 (Mature T-cells): 61% - 91% / 558 - 2061 cells/uL
CD4 (Helper T-cells): 32% - 65% / 487 - 1678 cells/uL
CD8 (Suppressor T-Cells): 13% - 39% / 201 - 1045 cells/uL
CD4/CD8 Ratio: 0.9 - 3.7

See CBC for its Reference Ranges
Critical Ranges:  
Test Comments: A complete Blood count will be reflexed and charged 
Methodology: Flow Cytometry
Clinical Significance:
To achieve subsequent CD4+ T-Cell counts after baseline values have previously been established.
Documentation: The CDC Guidelines for the performance of CD4+ T-Cell Determinations in persons with Human Immunodeficiency Virus Infection recommends the Lymphocyte Subset Panel to achieve baseline quantitations for T&B cell populations. MMWR Vol 41/No. RR-8, 1992.
Custom Panel: No


Turn Around Time: 48 to 72 hours
Days Performed: Monday, Wednesday, Friday, Saturday
Sites Performed: Parkview Regional Medical Center
PHL Test Code: FTCEL
EPIC Test Code: LAB342
Alternate Test Names: CD4; Flow Cytometry T Cell Subset; T Cell Subset with CBC; TCell Subset with CBC; T-Cell Subset with CBC
Included Tests: CD3; CD4; CD8; Helper/Suppressor Ratio; CBC [Automated Differential; Hematocrit (Hct); Hemoglobin (Hgb); Mean Corpuscular Hemoglobin (MCH); Mean Corpuscular Hemoglobin Concentration (MCHC); Mean Corpuscular Volume (MCV); Platelet (PLT);  Red Blood Cell Count (RBC); Red Cell Distribution Width Standard Deviation (RDWSD);  Red Cell Distribution Width Coefficient Variation (RDWCV); White Blood Cell Count (WBC]
CPT Coding: 86359, 86360, 85025 (Reflex 85007, 85008)

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