Last Modified:
8/21/2023 1:45:01 PM
Medical Necessity Documentation:
Client Notes:
CBCWD must be run within 24 hours of FTCEL testing
Patient Preparation:
Specimen Requirements:
3.0 mL Whole Blood in a Green Top Tube - Na Heparin
and
3.0 mL Whole Blood in a Lavender Top Tube - EDTA
Collection Instructions:
Transport & Storage: Temperature/Stability:
48 hours Ambient
Rejection Criteria:
Specimens with Lithium Heparin; Specimen suitability will be determined by the flow cytometry department only
Reference Range:
Pediatric (less than 3 years)
CD3 (Mature T-cells): 55% - 82% / 2324 - 3295 cells/uL
CD4 (Helper T-cells): 38% - 46% / 1538 - 2213 cells/uL
CD8 (Suppressor T-Cells): 8% - 31% / 351 - 2479 cells/uL
CD4/CD8 Ratio: 1.2 - 6.2
Adult
CD3 (Mature T-cells): 61% - 91% / 558 - 2061 cells/uL
CD4 (Helper T-cells): 32% - 65% / 487 - 1678 cells/uL
CD8 (Suppressor T-Cells): 13% - 39% / 201 - 1045 cells/uL
CD4/CD8 Ratio: 0.9 - 3.7
See CBC for its Reference Ranges
Critical Ranges:
Test Comments:
A complete Blood count will be reflexed and charged
Methodology:
Flow Cytometry
Clinical Significance:
To achieve subsequent CD4+ T-Cell counts after baseline values have previously been established.
Documentation:
The CDC Guidelines for the performance of CD4+ T-Cell Determinations in persons with Human Immunodeficiency Virus Infection recommends the Lymphocyte Subset Panel to achieve baseline quantitations for T&B cell populations. MMWR Vol 41/No. RR-8, 1992.
Custom Panel:
No
PRODUCTION SCHEDULE
Turn Around Time:
48 to 72 hours
Days Performed:
Monday, Wednesday, Friday, Saturday
Sites Performed:
Parkview Regional Medical Center
Other Testing Facility:
to Quest
PHL Test Code:
FTCEL
EPIC Test Code:
LAB342
Alternate Test Names:
CD4; Flow Cytometry T Cell Subset; Lymph Subset (CD4/TCELL); T Cell Subset with CBC; TCell Subset with CBC; T-Cell Subset with CBC
Included Tests:
CD3; CD4; CD8; Helper/Suppressor Ratio; CBC [Automated Differential; Hematocrit (Hct); Hemoglobin (Hgb); Mean Corpuscular Hemoglobin (MCH); Mean Corpuscular Hemoglobin Concentration (MCHC); Mean Corpuscular Volume (MCV); Platelet (PLT); Red Blood Cell Count (RBC); Red Cell Distribution Width Standard Deviation (RDWSD); Red Cell Distribution Width Coefficient Variation (RDWCV); White Blood Cell Count (WBC]
CPT Coding:
86359, 86360, 85025 (Reflex 85007, 85008)
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