HIV-1 Integrase Genotype

Overview

  • EPIC Code:
  • IMO1957
  • Soft Test Code:
  • HIVI
  • Send Out Test Code:
  • 16868
Alternate Names
  • Elvitegravir
  • HIV Integrase Genotype
  • HIV-1 Integrase Mutations
  • HIV-1 Integrase Strand Transfer Inhibitor
  • Isentress (Raltegravir, MK-0518) Resistance
  • MK-0518
  • Raltegravir
Clinical Significance

The emergence of integrase drug resistance mutations has been observed in vitro and in patients experiencing virologic failure on raltegravir in clinical trials. Twenty three percent of patients receiving raltegravir in a clinical trial experienced virologic failure at 48 weeks and genotypic analysis detected raltegravir associated resistance mutations in 68% of virologic failures. This assay amplifies and sequences the HIV-1 integrase gene and reports mutations at positions associated with integrase inhibitor drug resistance.


Specimen Collection & Preparation

Specimen Requirements

2.0 mL FROZEN Plasma from a Lavender Top Tube - EDTA in a Plastic Vial


Transport And Storage

24 hours Ambient
5 days Refrigerated
35 days Frozen (Preferred)

Ship frozen.


Collection Instructions

Value of last viral load and date of last viral load must be provided with the order

Separate plasma from the cells by centrifugation within 24 hours after collection.


Minimum Volume

0.6 mL Plasma


Neonatal Volume

Clinical Interpretation

Reference Range:

Refer to Interpretive Results


Methodology:
  • Reverse Transcriptase Polymerase Chain Reaction (RT-PCR)
  • Sequencing
Clinical Significance

The emergence of integrase drug resistance mutations has been observed in vitro and in patients experiencing virologic failure on raltegravir in clinical trials. Twenty three percent of patients receiving raltegravir in a clinical trial experienced virologic failure at 48 weeks and genotypic analysis detected raltegravir associated resistance mutations in 68% of virologic failures. This assay amplifies and sequences the HIV-1 integrase gene and reports mutations at positions associated with integrase inhibitor drug resistance.


Production Schedule

Sites Performed
  • Quest - Chantilly to San Juan Capistrano
Days Performed
Monday
Friday
Departments
  • Sendouts - Clinical
Turn Around Time

5 to 8 days


Coding & Compliance

CDM

00912601


CPT Coding

87906