Parkview Health Laboratory: Test Directory

Medical Necessity Documentation:

Patient Preparation:

Specimen Requirements:

4.0 mL Whole Blood in a Green Top Tube - Na Heparin
and
3.0 mL Whole Blood in a Lavender Top Tube - EDTA

Collection Instructions:

Transport & Storage: Temperature/Stability: 48 hours Ambient

Rejection Criteria: Specimens collected with Lithium Heparin; Specimen suitability will be determined by the flow cytometry department only

Reference Range:

Pediatric (less than 3 years)
CD3 (Mature T-cells): 55% - 82% / 2324 - 3295 cells/uL
CD4⁺/ CD3⁺(Helper T-cells): 38% - 46% / 1538 - 2213 cells/uL
CD8⁺ / CD3⁺ (Suppressor T-Cells): 8% - 31% / 351 - 2479 cells/uL
Helper/Suppressor Ratio: 1.2 - 6.2
CD 19 (B-cells): 11% - 45% / 430 - 3300 cells/uL
CD 56 (NK cells): 0% - 16% / 0 - 1200 cells/uL

Adult

CD3 (Mature T-cells): 61% - 91% / 558 - 2061cells/uL
CD4 (Helper T-cells): 32% - 65% / 487 - 1678 cells/uL

CD8 (Suppressor T-Cells): 13% - 39% / 201 - 1045 cells/uL

Helper/Suppressor Ratio: 0.9 - 3.7
CD 19 (B-cells): 4% - 25% / 30 - 550 cells/uL
CD 56 (NK cells): 1% - 29% / 0 - 800 cell/uL

See CBC for its Reference Ranges

Critical Ranges:

Test Comments: Complete Blood Count with differential (cpt 85025) maybe be reflexed and charged

Methodology: Flow Cytometry

Clinical Significance:

Documentation:

Custom Panel: No

Turn Around Time: 48 to 72 hours

Days Performed: Monday, Wednesday, Friday

Sites Performed: Parkview Regional Medical Center

PHL Test Code: FLSUB

EPIC Test Code: LAB2458

Alternate Test Names: Flow Cytometry Lymph Subset; Lymph Subset; Lymphocyte Immuonphenotyping; Lymphocyte Subset with CBC; Lymphocyte Subset, T & B Cell; Lymphocyte Subset, T Cell & B Cell; Natural Killer Cells; NK Cells; T & B Cell Subset

Included Tests: CD3; CD4; CD8; CD19; CD56; Helper/Suppressor Ratio; CBC [Hematocrit (Hct); Hemoglobin (Hgb); Mean Corpuscular Hemoglobin (MCH); Mean Corpuscular Hemoglobin Concentration (MCHC); Mean Corpuscular Volume (MCV); Platelet (PLT); Red Blood Cell Count (RBC); Red Cell Distribution Width Standard Deviation (RDWSD); Red Cell Distribution Width Coefficient Variation (RDWCV); White Blood Cell Count (WBC); WBC Differential]

CPT Coding: 85025 (Reflex 85007/85008) 86355, 86357, 86359, 86360

Parkview Laboratory: Test Directory

Lymphocyte Subset Panel

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