Epstein Barr Virus DNA, Qualitative

Overview

  • EPIC Code:
  • LAB2722
  • Soft Test Code:
  • EBVPC
  • Send Out Test Code:
  • 34179
Alternate Names
  • EBV DNA, QL PCR,Infectious Mononucleosis,EBV,PTLD,Post transplant
  • EBV DNA, Qual PCR
  • Epstein Barr Virus DNA, Qualitative, Real-Time PCR
  • Epstein-Barr Virus DNA, Qualitative
  • Lymphoproliferative
Clinical Significance

Infection with EBV is common and is generally subclinical or presents as a self-limited illness. Reactivation of latent EBV in an immunocompromised person can lead to more serious results, including lymphoproliferative disorders or neurological disease. PCR methods may be useful in identifying EBV in a variety of clinical specimens.


Specimen Collection & Preparation

Client Notes

If multiple Fluid or CSF test are being requested please specify order of importance


Specimen Requirements:

-OR-

1.0 mL Whole Blood in a Lavender Top Tube - EDTA
Minimum Volume:
0.3 mL Whole Blood*

* This volume does not allow for repeat testing

Rejection Criteria:

Frozen

Hemolyzed

Blood collected in tubes with heparin anticoagulant is not acceptable for this test


-OR-

1.0 ml serum in an SST Gold Top Tube
Minimum Volume:
0.3 mL Serum*

* This volume does not allow for repeat testing

Processing Instructions:

Allow blood to clot at room temperature and separate serum from cells within 2 hours of collection.

Transfer serum to sterile, plastic, screw-capped, plastic vial aliquot tube. 


Transport and Storage:
  • Ambient: 48 Hours

    Store acceptable

  • Refrigerated: 8 Days

    Store

    Transport 

  • Frozen (-20 C or colder): 30 Days

Specimen can be stored Ambient or Frozen

-OR-

1.0 mL CSF in a Sterile Plastic Leak-proof Contanier
Minimum Volume:
0.3 mL CSF*

* This volume does not allow for repeat testing

Collection Instructions:

Collect in a sterile, plastic container


Transport and Storage:
  • Ambient: 48 Hours
  • Refrigerated: 8 Days

    Store

    Transport 

  • Frozen (-20 C or colder): 30 Days

    Store

-OR-

> 3 cubic mm Fresh Tissue In a Sterile Plastic Leak-proof Container
Collection Instructions:

Collect in a sterile, plastic container


Transport and Storage:
  • Ambient: 48 Hours
  • Refrigerated: 8 Hours
  • Frozen (-20 C or colder): 30 Days

    Store

    Transport 

-OR-

1.0 mL Amniotic Fluid in Sterile Plastic Container
Minimum Volume:
0.3 mL Amniotic Fluid*

* This volume does not allow for repeat testing

Collection Instructions:

Collect in sterile plastic container


Transport and Storage:
  • Ambient: 48 Hours
  • Refrigerated: 8 Days

    Store

    Transport 

  • Frozen (-20 C or colder): 30 Days

-OR-

1.0 mL Ocular Fluid in Sterile Plastic Container
Minimum Volume:
0.3 mL Ocular Fluid*

* This volume does not allow for repeat testing

Collection Instructions:

Collect in sterile plastic container


Transport and Storage:
  • Ambient: 48 Hours
  • Refrigerated: 8 Days

    Store

    Transport 

  • Frozen (-20 C or colder): 30 Days

-OR-

1.0 mL Bronchoalveolar lavage (BAL) in Sterile Plastic Container
Minimum Volume:
0.3 mL Bronchoalveolar lavage (BAL) *

* This volume does not allow for repeat testing

Collection Instructions:

Collect in sterile plastic container


Transport and Storage:
  • Ambient: 48 Hours
  • Refrigerated: 8 Days

    Store

    Transport 

  • Frozen (-20 C or colder): 30 Days

-OR-

1.0 mL Bronchial Wash in Sterile Plastic Container
Minimum Volume:
0.3 mL Bronchial Wash*

* This volume does not allow for repeat testing

Collection Instructions:

Collect in sterile plastic container


Transport and Storage:
  • Ambient: 48 Hours
  • Refrigerated: 8 Days

    Store

    Transport 

  • Frozen (-20 C or colder): 30 Days

-OR-

1.0 mL Tracheal Secretions in Sterile Plastic Container
Minimum Volume:
0.3 mL Tracheal Secretions*

* This volume does not allow for repeat testing

Collection Instructions:

Collect in sterile plastic container


Transport and Storage:
  • Ambient: 48 Hours
  • Refrigerated: 8 Days

    Store

    Transport 

  • Frozen (-20 C or colder): 30 Days

Clinical Interpretation

Reference Range:

Not detected


Methodology:
  • Real-Time Polymerase Chain Reaction
Clinical Significance

Infection with EBV is common and is generally subclinical or presents as a self-limited illness. Reactivation of latent EBV in an immunocompromised person can lead to more serious results, including lymphoproliferative disorders or neurological disease. PCR methods may be useful in identifying EBV in a variety of clinical specimens.


Production Schedule

Sites Performed
  • Quest - Chantilly
Days Performed
Sunday
Monday
Tuesday
Wednesday
Thursday
Friday
Saturday
Departments
  • Sendouts - Clinical
Turn Around Time

3 to 6 days


Coding & Compliance

CDM

00910635


CPT Coding

87798