Reverse Transcriptase-PCR (RT-PCR) is a highly sensitive method used to detect Enterovirus (EV) RNA from various clinical specimens. This assay targets a conserved region of the EV genome which allows detection of nearly the entire spectrum of human enteroviruses, including the coxsackieviruses, polioviruses and the echoviruses. It does not differentiate among the EV serotypes. The diagnosis of enterovirus infection should not rely solely upon the result of a RT-PCR assay. A positive result should be considered in conjunction with clinical presentation and additional established clinical tests. A negative RT-PCR result indicates the absence of enterovirus RNA in the sample tested and does not exclude the diagnosis of disease.
Room temperature: 48 hours
Refrigerated: 7 days
Frozen: 30 days
Specimens can be stored and transported either refrigerated or frozen. Avoid repeated freezing and thawing of specimens.
0.3 mL CSF
Not detected
Reverse Transcriptase-PCR (RT-PCR) is a highly sensitive method used to detect Enterovirus (EV) RNA from various clinical specimens. This assay targets a conserved region of the EV genome which allows detection of nearly the entire spectrum of human enteroviruses, including the coxsackieviruses, polioviruses and the echoviruses. It does not differentiate among the EV serotypes. The diagnosis of enterovirus infection should not rely solely upon the result of a RT-PCR assay. A positive result should be considered in conjunction with clinical presentation and additional established clinical tests. A negative RT-PCR result indicates the absence of enterovirus RNA in the sample tested and does not exclude the diagnosis of disease.
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