Overview
EPIC Code:
- LAB2639
Soft Test Code:
- MSOT
Send Out Test Code:
- 15082
Alternate Names
- Coxsackie Virus PCR
- D68
- Echovirus PCR
- Enterovirus RNA, Qualitative Real-Time PCR
Clinical Significance
Reverse Transcriptase-PCR (RT-PCR) is a highly sensitive method used to detect Enterovirus (EV) RNA from various clinical specimens. This assay targets a conserved region of the EV genome which allows detection of nearly the entire spectrum of human Enteroviruses, including the Coxsackieviruses, Polioviruses and the Echoviruses. It does not differentiate among the EV serotypes or differentiate EV from rhinovirus. The diagnosis of Enterovirus infection should not rely solely upon the result of a RT-PCR assay. A positive result should be considered in conjunction with clinical presentation and additional established clinical tests. A negative RT-PCR result indicates the absence of Enterovirus RNA in the sample tested and does not exclude the diagnosis of disease.
Specimen Collection & Preparation
Specimen Requirements:
-OR-
0.7 mL CSF in a Sterile Container
Minimum Volume:
0.3 mL CSF** This volume does not allow for repeat testing
Transport and Storage:
-
Ambient (18-24°C):
48 Hours
-
Refrigerated (2-8°C):
7 Days
Transport (Cold Packs)
-
Frozen (-20° C or colder):
30 Days
Do not use frost free units that undergo repeated freeze/thaw cycles.
-OR-
1.0 mL Serum from a Red Top Tube in a Plastic Vial
Minimum Volume:
0.3 mL Serum** This volume does not allow for repeat testing
Rejection Criteria:
Heparinized specimens
Transport and Storage:
-
Ambient (18-24°C):
48 Hours
-
Refrigerated (2-8°C):
7 Days
Transport (Cold Packs)
-
Frozen (-20° C or colder):
30 Days
Do not use frost free units that undergo repeated freeze/thaw cycles.
-OR-
1.0 mL Plasma from a Lavender Top Tube - EDTA in a Plastic Vial
Minimum Volume:
0.5 mL Plasma** This volume does not allow for repeat testing
Rejection Criteria:
Blood collected in tubes with heparin anticoagulant is not accepted for this test
Heparinized specimens
Transport and Storage:
-
Ambient (18-24°C):
4 Days
Whole Blood stored
-
Refrigerated (2-8°C):
7 Days
Transport (Cold Packs)
-
Frozen (-20° C or colder):
30 Days
Do not use frost free units that undergo repeated freeze/thaw cycles.
-OR-
0.3 mL Pericardial Fluid in a Sterile Plastic Vial
Minimum Volume:
0.25 mL Pericardial Fluid** This volume does not allow for repeat testing
Collection Instructions:
Collect in a sterile, leak proof, plastic container
Rejection Criteria:
Heparinized specimens
Transport and Storage:
-
Ambient (18-24°C):
48 Hours
-
Refrigerated (2-8°C):
7 Days
Transport (Cold Packs)
-
Frozen (-20° C or colder):
30 Days
Do not use frost free units that undergo repeated freeze/thaw cycles.
-OR-
1 gram or 0.7 mL Frozen Stool in a Sterile Container
Specimen Information:
Transport and Storage:
-
Frozen (-20° C or colder):
Transport
-OR-
Rectal Swab in a M4 Media
Specimen Information:
Transport and Storage:
-
Ambient (18-24°C):
48 Hours
-
Refrigerated (2-8°C):
7 Days
Transport (Cold Packs)
-
Frozen (-20° C or colder):
30 Days
Do not use frost free units that undergo repeated freeze/thaw cycles.
-OR-
Rectal Swab in a Viral Culture Media (VCM )
Specimen Information:
Transport and Storage:
-
Ambient (18-24°C):
48 Hours
-
Refrigerated (2-8°C):
7 Days
Transport (Cold Packs)
-
Frozen (-20° C or colder):
30 Days
Do not use frost free units that undergo repeated freeze/thaw cycles.
-OR-
Rectal Swab in a Universal Transport Media (UTM)
Specimen Information:
Transport and Storage:
-
Ambient (18-24°C):
48 Hours
-
Refrigerated (2-8°C):
7 Days
Transport (Cold Packs)
-
Frozen (-20° C or colder):
30 Days
Do not use frost free units that undergo repeated freeze/thaw cycles.
-OR-
Throat Swab in a M4 Media
Specimen Information:
Transport and Storage:
-
Ambient (18-24°C):
48 Hours
-
Refrigerated (2-8°C):
7 Days
Transport (Cold Packs)
-
Frozen (-20° C or colder):
30 Days
Do not use frost free units that undergo repeated freeze/thaw cycles.
-OR-
Throat Swab in a Viral Culture Media (VCM )
Specimen Information:
Transport and Storage:
-
Ambient (18-24°C):
48 Hours
-
Refrigerated (2-8°C):
7 Days
Transport (Cold Packs)
-
Frozen (-20° C or colder):
30 Days
Do not use frost free units that undergo repeated freeze/thaw cycles.
-OR-
Throat Swab in a Universal Transport Media (UTM)
Specimen Information:
Transport and Storage:
-
Ambient (18-24°C):
48 Hours
-
Refrigerated (2-8°C):
7 Days
Transport (Cold Packs)
-
Frozen (-20° C or colder):
30 Days
Do not use frost free units that undergo repeated freeze/thaw cycles.
-OR-
Lesion Swab in a M4 Media
Specimen Information:
Transport and Storage:
-
Ambient (18-24°C):
48 Hours
-
Refrigerated (2-8°C):
7 Days
Transport (Cold Packs)
-
Frozen (-20° C or colder):
30 Days
Do not use frost free units that undergo repeated freeze/thaw cycles.
-OR-
Lesion in a Viral Culture Media (VCM )
Specimen Information:
Transport and Storage:
-
Ambient (18-24°C):
48 Hours
-
Refrigerated (2-8°C):
7 Days
Transport (Cold Packs)
-
Frozen (-20° C or colder):
30 Days
Do not use frost free units that undergo repeated freeze/thaw cycles.
-OR-
Lesion Swab in a Universal Transport Media (UTM)
Specimen Information:
Transport and Storage:
-
Ambient (18-24°C):
48 Hours
-
Refrigerated (2-8°C):
7 Days
Transport (Cold Packs)
-
Frozen (-20° C or colder):
30 Days
Do not use frost free units that undergo repeated freeze/thaw cycles.
-OR-
Nasopharyngeal Swab in a M4 Media
Specimen Information:
Transport and Storage:
-
Ambient (18-24°C):
48 Hours
-
Refrigerated (2-8°C):
7 Days
Transport (Cold Packs)
-
Frozen (-20° C or colder):
30 Days
Do not use frost free units that undergo repeated freeze/thaw cycles.
-OR-
Nasopharyngeal Swab in a Viral Culture Media (VCM )
Specimen Information:
Transport and Storage:
-
Ambient (18-24°C):
48 Hours
-
Refrigerated (2-8°C):
7 Days
Transport (Cold Packs)
-
Frozen (-20° C or colder):
30 Days
Do not use frost free units that undergo repeated freeze/thaw cycles.
-OR-
Nasopharyngeal Swab in a Universal Transport Media (UTM)
Specimen Information:
Transport and Storage:
-
Ambient (18-24°C):
48 Hours
-
Refrigerated (2-8°C):
7 Days
Transport (Cold Packs)
-
Frozen (-20° C or colder):
30 Days
Do not use frost free units that undergo repeated freeze/thaw cycles.
Clinical Interpretation
Reference Range:
Not detected
Methodology:
- Real-Time Polymerase Chain Reaction
Clinical Significance
Reverse Transcriptase-PCR (RT-PCR) is a highly sensitive method used to detect Enterovirus (EV) RNA from various clinical specimens. This assay targets a conserved region of the EV genome which allows detection of nearly the entire spectrum of human Enteroviruses, including the Coxsackieviruses, Polioviruses and the Echoviruses. It does not differentiate among the EV serotypes or differentiate EV from rhinovirus. The diagnosis of Enterovirus infection should not rely solely upon the result of a RT-PCR assay. A positive result should be considered in conjunction with clinical presentation and additional established clinical tests. A negative RT-PCR result indicates the absence of Enterovirus RNA in the sample tested and does not exclude the diagnosis of disease.
Production Schedule
Sites Performed
- Quest - Chantilly
Days Performed
Monday
Tuesday
Wednesday
Thursday
Friday
Saturday
Departments
- Sendouts - Miscellaneous
Turn Around Time
1 to 3 days
Coding & Compliance
CDM
00913333
CPT Coding
87498