Enterovirus RNA, Qualitative (Other)

Overview

  • EPIC Code:
  • MISC
  • Soft Test Code:
  • MSOT
  • Send Out Test Code:
  • 15082
Clinical Significance

Reverse Transcriptase-PCR (RT-PCR) is a highly sensitive method used to detect Enterovirus (EV) RNA from various clinical specimens. This assay targets a conserved region of the EV genome which allows detection of nearly the entire spectrum of human enteroviruses, including the coxsackieviruses, polioviruses and the echoviruses. It does not differentiate among the EV serotypes. The diagnosis of enterovirus infection should not rely solely upon the result of a RT-PCR assay. A positive result should be considered in conjunction with clinical presentation and additional established clinical tests. A negative RT-PCR result indicates the absence of enterovirus RNA in the sample tested and does not exclude the diagnosis of disease.


Specimen Collection & Preparation

Specimen Requirements

0.7 mL CSF in a sterile container


Transport And Storage

Room temperature: 48 hours
Refrigerated: 7 days
Frozen: 30 days

Specimens can be stored and transported either refrigerated or frozen. Avoid repeated freezing and thawing of specimens.


Collection Instructions

 


Minimum Volume

0.3 mL CSF


Neonatal Volume

Clinical Interpretation

Reference Range:

Not detected


Methodology:
  • Real-Time Polymerase Chain Reaction
Clinical Significance

Reverse Transcriptase-PCR (RT-PCR) is a highly sensitive method used to detect Enterovirus (EV) RNA from various clinical specimens. This assay targets a conserved region of the EV genome which allows detection of nearly the entire spectrum of human enteroviruses, including the coxsackieviruses, polioviruses and the echoviruses. It does not differentiate among the EV serotypes. The diagnosis of enterovirus infection should not rely solely upon the result of a RT-PCR assay. A positive result should be considered in conjunction with clinical presentation and additional established clinical tests. A negative RT-PCR result indicates the absence of enterovirus RNA in the sample tested and does not exclude the diagnosis of disease.


Production Schedule

Sites Performed
  • Quest - Chantilly
Days Performed
Sunday
Monday
Tuesday
Wednesday
Thursday
Friday
Saturday
Departments
  • Sendouts - Miscellaneous

Coding & Compliance

CDM

00913333


CPT Coding

87498