Enterovirus RNA, Qualitative (Other)

Last Modified: 7/18/2016 8:10:24 AM

Specimen Requirements:
Serum 1.0 mL ref 7 days, frozen 30 days

Plasma 1.0 mL, ref 7days, Froz 30 days (ACD or EDTA)
Collect blood in sterile tubes containing EDTA or ACD as anticoagulant or in plasma preparation tubes (PPTs). Store collected whole blood at room temperature and separate plasma from cells within 2 hours of collection. Transfer plasma to sterile, plastic, screw-capped tubes and store refrigerated or frozen. If blood is collected in a PPT tube, centrifuge within 2 hours of collection and store refrigerated or frozen. It is not necessary to transfer the plasma from a PPT tube to aliquot tubes.

Rectal Swab in M4 , Ref 7 days,
Do not freeze swabs in VCM or M4 Transport Medium

Stool 0.5 mL, Ref 7 days, Froz 30 days (Frozen if stool specimen)

Throat Swab in M4, Ref 7 days,
Do not freeze swabs in VCM or M4 Transport Medium
Transport & Storage: Temperature/Stability: Room temperature: 48 hours
Refrigerated: 7 days
Frozen: 30 days

Specimens can be stored and transported either refrigerated or frozen. Avoid repeated freezing and thawing of specimens.
Rejection Criteria: Bronchial lavage or washing
Reference Range: Not detected
Methodology: Real-Time Polymerase Chain Reaction (RT-PCR)
Clinical Significance: Reverse Transcriptase-PCR (RT-PCR) is a highly sensitive method used to detect Enterovirus (EV) RNA from various clinical specimens. This assay targets a conserved region of the EV genome which allows detection of nearly the entire spectrum of human enteroviruses, including the coxsackieviruses, polioviruses and the echoviruses. It does not differentiate among the EV serotypes. The diagnosis of enterovirus infection should not rely solely upon the result of a RT-PCR assay. A positive result should be considered in conjunction with clinical presentation and additional established clinical tests. A negative RT-PCR result indicates the absence of enterovirus RNA in the sample tested and does not exclude the diagnosis of disease.
Custom Panel: No


Days Performed: Sunday, Monday, Tuesday, Wednesday, Thursday, Friday, Saturday
Sites Performed: Quest - Chantilly
PHL Test Code: MSOT
EPIC Test Code:
Send Out Test Code: 15082
CPT Coding: 87498

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