Coombs, Direct by Hemagglutination

Last Modified: 2/25/2020 11:52:02 AM

Medical Necessity Documentation:  
Client Notes:  
Patient Preparation:  
Specimen Requirements: 1.0 mL Whole Blood in a Lavender Top Tube - EDTA
Collection Instructions: Specimen should be labeled with the patient's name, date and time of collection, [date of birth and/or social security number] and the phlebotomist's (collector's) initials
Minimum Volume: 1.0 mL Whole Blood in Pink Top Tube
Neonatal Volume: Pediatric 1.0 mL Whole Blood in a Pink Microtainer
Transport & Storage: Temperature/Stability: 24 hours Ambient 
72 hours Refrigerated

This Blood Bank test should be performed within 72 hours. If longer than 72 hours patient should be redrawn.
Rejection Criteria: Serum Separator Tube (SST or PST)
Reference Range: Negative
Critical Ranges:  
Test Comments:
Direct Fractionated Coombs will be ordered and charged when result on patients 6 months and older is positive

HDN Antibody Screen will be ordered when the Direct Coombs Test is positive on a cord blood
Methodology: Hemagglutination or gel hemagglutination
Clinical Significance:  
Custom Panel: No


Stat Eligible: Yes
Days Performed: Sunday, Monday, Tuesday, Wednesday, Thursday, Friday, Saturday
Sites Performed: Parkview Dekalb, Parkview Hospital Randallia, Parkview Huntington, Parkview LaGrange, Parkview Noble, Parkview Regional Medical Center, Parkview Wabash, Parkview Whitley
PHL Test Code: DAT1
EPIC Test Code: LAB274
Alternate Test Names: Direct Antiglobulin Test; Direct Coombs
Included Tests:  
CPT Coding: 86880 (Reflexes 86880)

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