14-3-3 Protein, CSF (Creutzfeldt-Jakob)

Last Modified: 1/4/2024 12:51:04 PM


Medical Necessity Documentation:  
Client Notes:  
Patient Preparation: Patient must be 12 years of age or older.
Specimen Requirements: 2.0 mL CSF in a Sterile Plastic Vial by lumbar puncture

Collection Instructions: National Prion Order Form Clinical History required to be filled out by ordering provider 


Collect CSF by lumbar puncture.
Discard first 2 mL that flows from tap.
Collect next 2 mL CSF, avoiding bloody tap.
Freeze within 20 minutes of collection.

Minimum Volume: 0.6 mL CSF on ice
Transport & Storage: Temperature/Stability: Indefinetly days Frozen (transport) 

Rejection Criteria: Bloody and insufficient sample quantity
Reference Range:

 

For Non-Prion Disease

RT-QuIC: Negative
Total TAU (ELISA): 0-1149 pg/mL
14-3-3 GAMMA (ELISA): <173 - 1999 AU/mL.

Likelihood of Prion Disease

RT-QuIC Positive: >98%
RT-QuIC Negative & Total TAU <499 = <0.2%
RT-QuIC Negative & Total TAU 500-2499 = <1%

 

Critical Ranges:  N/A
Test Comments:  Freeze within 20 minutes of collection. Store and transport frozen. Ship using a styrofoam container with sufficient dry ice (5 lbs/24 hours). The NPDPSC requests a urine sample for research purposes with all CSF samples if available.
Methodology: Quantitative Enzyme-Linked Immunosorbent Assay (ELISA). Real-Time Quaking-Induced Conversion (RT-Qu
Clinical Significance:

May aid in diagnosis of prion disease.
Assumes that the patient has a history of a rapid neurodegenerative condition and does not have several conditionsi that might compromise the test results.

Documentation:
Custom Panel: No

PRODUCTION SCHEDULE

Turn Around Time: 7 days
Sites Performed: Quest - Chantilly to National Prion Lab
PHL Test Code: CJD
EPIC Test Code: LAB2785
Send Out Test Code: 37989
Alternate Test Names: 14-3-3 (ELISA), Total TAU (ELISA) and RT-QuIC Pane; 14-3-3 Protein, CSF; 14-3-3 Protein, Spinal Fluid; CJD; Cretuzfeld-Jakob; Creutzfeldt-Jakob 14-3-3 Protein; Protein 14-3-3
Included Tests:  
CPT Coding: 0035U; 83520 (x2)

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