14-3-3 Protein, CSF (Creutzfeldt-Jakob)

Overview

  • EPIC Code:
  • LAB2785
  • Soft Test Code:
  • CJD
  • Send Out Test Code:
  • 37989
Alternate Names
  • 14-3-3 (ELISA), Total TAU (ELISA) and RT-QuIC Panel
  • 14-3-3 Protein, CSF
  • 14-3-3 Protein, Spinal Fluid
  • CJD
  • Cretuzfeld-Jakob
  • Creutzfeldt-Jakob 14-3-3 Protein
  • Protein 14-3-3
Clinical Significance

May aid in diagnosis of prion disease.
Assumes that the patient has a history of a rapid neurodegenerative condition and does not have several conditionsi that might compromise the test results.


Specimen Collection & Preparation

Test Notes:

Patient must be 12 years of age or older.


Required Forms & Information:

Complete National Prion Disease Requisition

Clinical History required to be filled out by ordering provider 


Specimen Overview

If suspected CJD, CSF samples MUST be labeled as " Suspected CJD"  

For more information please refer to the Creutzfeldt-Jakob Disease policy. 

Due to stability time constraints avoid collections on Friday & Saturday

Specimen Requirements:

-OR-

2.0 mL Cerbrospinal Fluid (CSF) in Sterile Container
Collection Instructions:

Collect CSF by lumbar puncture
Discard first 2.0 mL that flows from tap
Collect next 2.0 mL to 5.0 mL CSF, avoiding bloody tap.


Processing Instructions:

Freeze within 20 minutes of collection


Rejection Criteria:

Bloody and insufficient sample quantity

Samples that are colored or contain blood cannot be performed for RT-QuIC and 14-3-3 GAMMA


Transport and Storage:
  • Ambient: Unacceptable
  • Refrigerated: Unacceptable
  • Frozen (-20 C or colder): Indefinetly

    The sample must be immediately frozen, at least in a -20° C freezer

    *** Ship Monday through Wednesday (priority overnight) ***

    Ship on dry ice using a styrofoam container with sufficient dry ice (5 lbs/24 hours).

Clinical Interpretation

Reference Range:

Refer to Interpretive Results 


Methodology:
  • Immunoassay (IA)
  • Real-Time Quaking-Induced Conversion (RT-QuIC)

Clinical Significance

May aid in diagnosis of prion disease.
Assumes that the patient has a history of a rapid neurodegenerative condition and does not have several conditionsi that might compromise the test results.


Production Schedule

Sites Performed
  • Quest - Chantilly to National Prion Lab
Days Performed
Tuesday
Thursday
Departments
  • Sendouts - Clinical
  • Blood Bank
Turn Around Time

7 days


Coding & Compliance

CDM

00913960 (x2); 00913959


CPT Coding

86317 (14-3-3 & TAU), 0035U (RT-QuIC)