Disseminated Intravascular Coagulation Panel

Included Tests

Specimen Requirements

Platelet Poor Plasma from a 2.7 mL Whole Blood in a Blue Top Tube - 3.2% Buffered Sodium Citrate in a Plastic Vial
and
2.7 mL Whole Blood in a Blue Top Tube -  3.2% Buffered Sodium Citrate
and
3.0 mL Whole Blood in 2 Lavender Top Tubes - EDTA

Alternate Specimen

1.0 mL Platelet Poor Plasma from a full Blue Top Tube - 3.2% Buffered Sodium Citrate in a Plastic Vial when unable to send to testing laboratory within the transport stability levels. Unfractionated heparinized patient 4 hour Ambient 

Non-heparinized patient 24 hours Ambient 

TransportAndStorage

Platelet Poor Plasma: 
Unfractionated heparinized patient 4 hour Ambient Blue

Non-heparinized patient 24 hours Ambient 

  This tube must be transported to testing laboratory within 45 minutes from collection. If this is not a possibility follow the forllowing steps: 
1. Heparinized Patient specimens must be spun wihtin 1 hour of collection, following these Platelet Poor Plasma preparation instructions 
2. Send the "frozen" platelet poor plasma & primary tube to the testing laboratory
3. Contact the laboratory at 266-1500 option 1, to notify PRMC laboratory that he specimen is coming

Blue Top Tube (whole blood):
Unfractionated heparinized patient 4 hour Ambient 

Non-heparinized patient 24 hours Ambient 

Lavendar Top Tube:
24 hours Ambient
72 hours Refrigerated

Collection Instructions

Blue Top Tube(s) Collection
Correct ratio of Blood to Citrate is critical (9:1). Specimens must be filled within +/- 10% of stated volume.Specimens must be filled appropriately see this example

If a Blue Top Tube is collected utilizing a butterfly, a Blue Discard Tube must be drawn first to remove air from the line. If this is not done, the Blue Top Tube will not be filled properly due to the vacuum in tube and a redraw will be required

*Heparinized Patient specimens must be spun within 1 hour of collection

Note: Collection of blood for coagulation testing through intravenous lines that have been previously flushed with heparin should be avoided, if possible. 
If the blood must be drawn through an indwelling catheter, possible heparin contamination and specimen dilution should be considered. 
When obtaining specimsn from indwelling lines that may contain heparin, the line should be flushed with 5mL of saline, and the first 5 mL of blood or 6-times the line volume
(dead space volume of the catheter) be drawn off and discarded before the coagulation tube is filled. For those samples collected from a normal saline lock (capped off venous port) twice the dead space volume of the catheter and extension set should be discarded.

Platelet Poor Plasma Blue Top 1 specific collection and processing instructions click here Platelet Poor Plasma 

Lavendar Top Tube
Mix tube by inversion 5 times

Do not centrifuge

Specimens must not be clotted and cannot contain microclots. Overfilling or underfilling may allow blood to clot. Specimens may be rejected due to WBC deterioration resulting from improper storage or age of specimen

For slide preparation see Addendum D

Minimum Volume

Neonatal Volumne

Reference Range:

Refer to Individual Tests

Methodology:

• Multiple - Based on Individual Components

CDM

01432899, 01495379, 01450040, 01450039, 01450046

CPT Coding

85049, 85379, 85384, 85610, 85730

Medical Necessity May Apply