Last Modified:
7/27/2022 8:36:34 AM
Medical Necessity Documentation:
Client Notes:
Patient Preparation:
Specimen Requirements:
3 - 7.0 mlL Whole Blood Red Top Tubes
and
2 - 5.0 mL Whole Blood in a Lavender Top Tubes - EDTA
Collection Instructions:
Transport & Storage: Temperature/Stability:
Reference Range:
Please Refer to interpretive report
Critical Ranges:
Test Comments:
Negative results do not completely rule out the presence of immune-mediated hemolytic anemia. Positive test results are not absolutely diagnostic of immune hemolytic anemia and must be used in combination with clinical information to make a diagnosis
Methodology:
Serological
Clinical Significance:
Evaluation of patients developing hemolysis in the absence of serologic evidence to support a diagnosis of immune-mediated hemolytic anemia. Results may allow diagnosis of autoimmune hemolytic anemia, thereby assisting the physician in management of the patient.
Documentation:
A negative Direct Antiglobulin Test (DAT) is seen in approximately 1% of patients presenting with signs and symptoms of immune hemolysis. A well-performed standard DAT detects approximately 100-500 molecules of bound IgG per red cell. Our laboratory performs a more sensitive DAT1 (“super Coombs”) in order to confirm a diagnosis of autoimmune hemolytic anemia in patients whose samples have tested negative in a standard DAT for any of the following reasons: • Patient RBCs are sensitized with small numbers of IgG molecules, below the level of detection of the standard DAT. • Low-affinity IgG autoantibodies are eluted from RBCs during routine washing of the RBCs prior to the detection phase of the standard DAT. • IgA autoantibodies not detected by a standard DAT that is performed using monoclonal AHG
Custom Panel:
No
PRODUCTION SCHEDULE
Turn Around Time:
3 days
Departments:
PHL Test Code:
MSOT
EPIC Test Code:
MISC
Send Out Test Code:
3111
Alternate Test Names:
DAT Negative Workup ; Immune Hemolytic Anemia Evaluation; Micro Coombs; Super Coombs
Included Tests:
ABO/Rh, Direct Antiglobulin Tests (including IgA detection); Elution (routine and enhanced); Antibody detection tests (routine and enhanced). additional tests as necessary based upon results determined by Immunohematology Reference Lab
CPT Coding:
86880x8, 86860, 86850x5, 86900, 86901, 86870
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