Histoplasma Antibody, IgG / IgM

Last Modified: 3/27/2019 3:42:29 PM

Medical Necessity Documentation:  
Client Notes:  
Patient Preparation:  
Specimen Requirements: 1.0 mL Serum from a Red Top Tube in a Plastic Vial
Collection Instructions: Collect serum specimens in serum separator or red top tube. Allow blood to clot for 30 minutes, then centrifuge. Pipette serum into a plastic screw cap vial.
Minimum Volume: 0.5 mL Serum
Transport & Storage: Temperature/Stability: 14 days Ambient
14 days Refrigerated
14 days Frozen
Rejection Criteria: > 14 days; Not Serum; Do not meet criteria required.
Reference Range: Negative
Critical Ranges:  
Test Comments: Negative: <8.0 EU

Intermediate: 8.0 EU-9.9 EU

Positive: 10.0 EU-80.0 EU

Methodology: Semi-Quantitative Indirect Enzyme Immunoassay (EIA)
Clinical Significance: IgM and IgG antibodies to Histoplasma antigen usually appear during the first month of infection. The IgM antibody response in acute pulmonary histoplasmosis is detectable during the acute phase (roughly 3 weeks) and is shown to decline during the convalescent stage (at about 6 weeks); whereas, IgG levels remained relatively constant at 6 weeks. Follow-up testing may be considered 2-4 weeks after initial testing to determine if antibody levels are increasing, especially in patients with low positive (10 EU – 20 EU) or intermediate results. Increase in IgG antibody or decrease in IgM concentration would suggest recent infection. Antibodies may persist for several years in patients with chronic pulmonary complications or progressive extrapulmonary (disseminated) histoplasmosis. Antibodies may be falsely-negative in some progressive or chronic cases, especially in immunocompromised patients. Antibodies may also be detected in healthy subjects who are asymptomatic as a result of sub-clinical infection within the last 18-36 months.
Documentation: Limitation
  • 12% cross reactivity with Blastomycosis
  • 24% cross reactivity with Coccidioidomycosis
  • Custom Panel: No


    Turn Around Time: 1 day
    Days Performed: Monday, Thursday
    Sites Performed: MiraVista
    PHL Test Code: HISAB
    EPIC Test Code: IMO4110
    Send Out Test Code: 326
    Alternate Test Names: Histoplasma AB IgG IgM EIA; Histoplasma Antibody, IgG & IgM; MVista® HISTOPLASMA ANTIBODY IgG IgM EIA
    Included Tests:  
    CPT Coding: 86698 x 2

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