Histoplasma Antigen Quantitative, Urine

Overview

  • EPIC Code:
  • LAB400
  • Soft Test Code:
  • UHIS2
  • Send Out Test Code:
  • 310
Alternate Names
  • Histoplasma Quantitative EIA Test
Clinical Significance

The MVista® histoplasma quantitative antigen test aids the diagnosis of histoplasmosis. Monitoring the histoplasmosis helps determine when treatment can be stopped and to diagnose relapse.

The result is not intended to be used as the sole means for the clinical diagnosis or patient management decisions.

  • Cross-reactions are seen with blastomycosis, paracoccidioidomycosis, penicilliosis, less frequently in coccidioidomycosis, rarely in aspergillosis and possibly sporotrichosis.
  • Sputolysin, sodium hydroxide and potassium hydroxide treatment degrade the analyte detected in the assay.


Specimen Collection & Preparation

Specimen Requirements:

-OR-

2.0 mL Urine in a STERILE Plastic Container
Minimum Volume:
0.5 mL Urine*

* This volume does not allow for repeat testing

Specimen Information:


Processing Instructions:

Identify specimen as urine on container


Rejection Criteria:

Stored in Transport Media, Fixative or Isolator Tubes


Transport and Storage:
  • Ambient: 2 Weeks
  • Refrigerated: 2 Weeks
  • Frozen (-20 C or colder): Indefinitely

-OR-

2.0 mL CSF in a STERILE Plastic Container
Minimum Volume:
0.8 mL CSF*

* This volume does not allow for repeat testing

Specimen Information:


Processing Instructions:

Identify specimen as CSF on container


Rejection Criteria:

If specimen is too viscous to pipette

Tissue, Sputum, Bronchial Brushings, Stool, FNA, Biopsy, Tracheal or Bone Marrow Aspirate

Stored in Transport Media, Fixative or Isolator Tubes


Transport and Storage:
  • Ambient: 2 Weeks
  • Refrigerated: 2 Weeks
  • Frozen (-20 C or colder): Indefinitely

-OR-

2.0 mL Bronchial Lavage Fluid (BAL) in a STERILE Plastic Container
Minimum Volume:
0.8 mL BAL*

* This volume does not allow for repeat testing

Specimen Information:


Processing Instructions:

Identify specimen as BAL on container


Rejection Criteria:

If specimen is too viscous to pipette

Tissue, Sputum, Bronchial Brushings, Stool, FNA, Biopsy, Tracheal or Bone Marrow Aspirate

Stored in Transport Media, Fixative or Isolator Tubes


Transport and Storage:
  • Ambient: 2 Weeks
  • Refrigerated: 2 Weeks
  • Frozen (-20 C or colder): Indefinitely

-OR-

2.0 mL Other Body Fluids in a STERILE Plastic Container
Minimum Volume:
0.8 mL BAL*

* This volume does not allow for repeat testing

Specimen Information:


Processing Instructions:

Identify specimen as body fluid type on container


Rejection Criteria:

If specimen is too viscous to pipette

Tissue, Sputum, Bronchial Brushings, Stool, FNA, Biopsy, Tracheal or Bone Marrow Aspirate

Stored in Transport Media, Fixative or Isolator Tubes


Transport and Storage:
  • Ambient: 2 Weeks
  • Refrigerated: 2 Weeks
  • Frozen (-20 C or colder): Indefinitely

Clinical Interpretation

Reference Range:

Non Detected


Test Comments:

Positive: 0.20-20.00 ng/mL

Positive Above the Limit of Quantification: Results greater than 20.00 ng/mL fall outside the linear range of the assay. These results are positive, but not accurately quantifiable.


Methodology:
  • Quantitative Sandwich Enzyme Immunoassay (EIA)
  • Serum and CSF are pre-treated to improve sensativity
Clinical Significance

The MVista® histoplasma quantitative antigen test aids the diagnosis of histoplasmosis. Monitoring the histoplasmosis helps determine when treatment can be stopped and to diagnose relapse.

The result is not intended to be used as the sole means for the clinical diagnosis or patient management decisions.

  • Cross-reactions are seen with blastomycosis, paracoccidioidomycosis, penicilliosis, less frequently in coccidioidomycosis, rarely in aspergillosis and possibly sporotrichosis.
  • Sputolysin, sodium hydroxide and potassium hydroxide treatment degrade the analyte detected in the assay.


Documentation

*New positives may require confirmation 


Production Schedule

Sites Performed
  • MiraVista
Days Performed
Monday
Tuesday
Wednesday
Thursday
Friday
Departments
  • Sendouts - Clinical
Turn Around Time

1 to 3 days


Coding & Compliance

CDM

00917110


CPT Coding

87385