FLT3 Mutation Analysis, Whole Blood

Overview

  • EPIC Code:
  • Soft Test Code:
  • FLT3B
  • Send Out Test Code:
  • 39786
Alternate Names
  • Acute Myeloid Leukemia
  • AML
  • fms-like tyrosine kinase 3
  • ITD
  • TKD
Clinical Significance

FLT3 (fms-like tyrosine kinase-3) is a receptor tyrosine kinase that plays a significant role in cell survival, proliferation, and differentiation of hematopoietic stem cells. Mutations of FLT3 represent one of the most frequent molecular mutations in acute myeloid leukemia (AML) and are reported in 25-30% of the patients. These mutations predominantly include FLT3 internal tandem duplications (ITD) which occur in the region of the gene encoding the juxtamembrane region of the protein and point mutations in the tyrosine kinase domain (TKD). The TKD mutations most frequently involve mutation or deletion of codons D835 or I836. Both ITD and TKD mutations constitutively activate FLT3 kinase activity without the need for the ligand and hence promote malignancy. AML patients with FLT3 internal tandem duplication (ITD) mutations have poor prognosis, high relapse rates, and reduced overall survival. Patients with FLT3 mutations could be eligible for FLT3-targeted therapy regimens.


Specimen Collection & Preparation

Specimen Requirements

5.0 mL Whole Blood in a Lavender Top Tube - EDTA


Transport And Storage

7 days Ambient
7 days Refrigerated (transport)


Collection Instructions

 


Minimum Volume

0.3 mL Whole Blood


Neonatal Volume

Clinical Interpretation

Reference Range:

FLT3 ITD:        Not detected

FLT3 TKD:       Not detected


Methodology:
  • Polymerase Chain Reaction (PCR)
Clinical Significance

FLT3 (fms-like tyrosine kinase-3) is a receptor tyrosine kinase that plays a significant role in cell survival, proliferation, and differentiation of hematopoietic stem cells. Mutations of FLT3 represent one of the most frequent molecular mutations in acute myeloid leukemia (AML) and are reported in 25-30% of the patients. These mutations predominantly include FLT3 internal tandem duplications (ITD) which occur in the region of the gene encoding the juxtamembrane region of the protein and point mutations in the tyrosine kinase domain (TKD). The TKD mutations most frequently involve mutation or deletion of codons D835 or I836. Both ITD and TKD mutations constitutively activate FLT3 kinase activity without the need for the ligand and hence promote malignancy. AML patients with FLT3 internal tandem duplication (ITD) mutations have poor prognosis, high relapse rates, and reduced overall survival. Patients with FLT3 mutations could be eligible for FLT3-targeted therapy regimens.


Production Schedule

Sites Performed
  • Quest - Chantilly
Days Performed
Sunday
Monday
Tuesday
Wednesday
Thursday
Friday
Saturday
Departments
  • Sendouts - Clinical
Turn Around Time

3 to 4 days


Coding & Compliance

CPT Coding

81245, 81246