25-Hydroxyvitamin D (D2, D3)

Last Modified: 4/26/2022 12:13:25 PM

Medical Necessity Documentation:  
Client Notes:  
Patient Preparation: Fasting preferred, but not required
Specimen Requirements: 1.0 mL Serum from a Red Top Tube in a Plastic Vial
Collection Instructions: Allow blood to clot at room temperature. Centrifuge and separate the serum from the cells immediately.
Transport & Storage: Temperature/Stability: 21 days Ambient - Transport
21 days Refrigerated
21 days Frozen

Rejection Criteria: Gross hemolysis • Gross lipemia • Heparinized or EDTA plasma • Serum not separated from Serum Separator Tube (SST®) gel or clot within 48 hours • Grossly icteric
Reference Range:
Vitamin D, 25-OH, Total 30-100 ng/mL
Vitamin D, 25-OH, D3 Not established
Vitamin D, 25-OH, D2 Not established
Critical Ranges:  
Test Comments:  
Methodology: Liquid Chromatography/Tandem Mass Spectrometry (LC/MS/MS)
Clinical Significance: Measurement of serum 25-OH vitamin D concentrations provide a good index of circulating vitamin D activity in patients not suffering from renal disease. Lower than normal 25-OH vitamin D levels can result from a dietary deficiency, poor absorption of the vitamin or impaired metabolism of the sterol in the liver. A 25-OH vitamin D deficiency can lead to bone diseases such as rickets and osteomalacia. Above normal levels can lead to hypercalcemia. This assay employs liquid chromatography tandem mass spectrometry to independently measure and report the two common forms of 25-hydroxy vitamin D: 25-OH D3 - the endogenous form of the vitamin and 25-OH D2 - the analog form used to treat 25-OH Vitamin D3 deficiency.
Custom Panel: No


Turn Around Time: 2 to 3 days
Days Performed: Monday, Tuesday, Wednesday, Thursday, Friday, Saturday
Sites Performed: Quest - Chantilly
PHL Test Code: MSOT
EPIC Test Code: LAB2524
Send Out Test Code: 92888
Alternate Test Names: Quest Assure D; Quest Assure D 25-Hydroxyvitamin D (D2,D3), LC/MS/; Quest Assure D™ 25-Hydroxyvitamin D (D2, D3), LC/M; QuestAssure D 25-Hydroxyvitamin D (D2,D3), LC/MS/M; QuestAssureD™ 25-Hydroxyvitamin D (D2, D3), LC/MS/; Vit D LC/MS/MS; Vitamin D LC/MS/MS; Vitamin D, 25 OH Total, D2, D3; Vitamin D, 25-OH, LC/MS/MS; Vitamin D2, 25-hydroxy; Vitamin D3, 25-hydroxy
Included Tests:

Vitamin D, 25-OH, Total; Vitamin D, 25-OH, D3; Vitamin D, 25-OH, D2

CPT Coding: 82306

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