Overview
EPIC Code:
- LAB2948
Soft Test Code:
- CMVQL
Send Out Test Code:
- 10601
Alternate Names
- CMV DNA, Qualitative
- CMV Qual PCR-Newborn
- Cytomegalovirus DNA, Qualitative Real-Time PCR
Clinical Significance
CMV infections are common and usually asymptomatic. In patients who are immunocompromised, CMV may cause
disseminated, severe disease. CMV may cause birth defects in a minority of infected newborns. DNA methods provide the highest sensitivity and specificity of any method.
Specimen Collection & Preparation
Specimen Requirements:
-OR-
1.0 mL Whole Blood in Lavender Top Tubes - EDTA
Minimum Volume:
0.5 ML Whole Blood** This volume does not allow for repeat testing
Collection Instructions:
Collect whole blood in sterile tubes containing EDTA as anticoagulant.
Rejection Criteria:
Blood collected in tubes with heparin anticoagulant
Frozen Specimen
Heparinized specimen
Transport and Storage:
-
Ambient (18-24°C):
48 Hours
-
Refrigerated (2-8°C):
8 Days
Store
Transport (Cold Packs)
-
Frozen (-20° C or colder):
Unacceptable
-OR-
1.0 mL Plasma from a Lavender Top Tube - EDTA in a Plastic Vial
Minimum Volume:
0.5 mL Plasma** This volume does not allow for repeat testing
Collection Instructions:
Collect blood in sterile tubes containing EDTA as anticoagulant.
Processing Instructions:
Centrifuge and separate plasma from cells within 2 hours of collection
Rejection Criteria:
Blood collected in tubes with heparin anticoagulant is not accepted for this test
Heparinized specimens
Transport and Storage:
-
Ambient (18-24°C):
4 Days
Whole Blood stored
-
Refrigerated (2-8°C):
7 Days
Store
Transport (Cold Packs)
-
Frozen (-20° C or colder):
30 Days
Store
-OR-
1.0 mL Serum from a Red Top Tube in a Plastic Vial
Minimum Volume:
0.3 mL Serum** This volume does not allow for repeat testing
Rejection Criteria:
Heparinized specimens
Transport and Storage:
-
Ambient (18-24°C):
48 Hours
-
Refrigerated (2-8°C):
8 Days
Store
Transport (Cold Packs)
-
Frozen (-20° C or colder):
30 Days
Store
-OR-
0.3 mL CSF in a Sterile Plastic Vial
Minimum Volume:
0.25 mL CSF** This volume does not allow for repeat testing
Collection Instructions:
Collect in a sterile, leak proof, plastic container
Transport and Storage:
-
Ambient (18-24°C):
48 Hours
-
Refrigerated (2-8°C):
8 Days
Store
Transport (Cold Packs)
-
Frozen (-20° C or colder):
30 Days
Store
-OR-
0.3 mL Amniotic Fluid in a Sterile Plastic Vial
Minimum Volume:
0.25 mL Amiotic Fluid** This volume does not allow for repeat testing
Collection Instructions:
Collect in a sterile, leak proof, plastic container
Rejection Criteria:
Heparinized specimens
Transport and Storage:
-
Ambient (18-24°C):
48 Hours
-
Refrigerated (2-8°C):
8 Days
Store
Transport (Cold Packs)
-
Frozen (-20° C or colder):
30 Days
Store
-OR-
0.3 mL Amniotic Fluid Supernate in a Sterile Plastic Vial
Minimum Volume:
0.25 mL Amiotic Fluid Supernate** This volume does not allow for repeat testing
Collection Instructions:
Collect in a sterile, leak proof, plastic container
Rejection Criteria:
Heparinized specimens
Transport and Storage:
-
Ambient (18-24°C):
48 Hours
-
Refrigerated (2-8°C):
8 Days
Store
Transport (Cold Packs)
-
Frozen (-20° C or colder):
30 Days
Store
-OR-
0.3 mL Bronchoalveolar Lavage (BAL) in a Sterile Plastic Vial
Minimum Volume:
0.25 mL Bronchoalveolar Lavage (BAL** This volume does not allow for repeat testing
Collection Instructions:
Collect in a sterile, leak proof, plastic container
Rejection Criteria:
Heparinized specimens
Transport and Storage:
-
Ambient (18-24°C):
48 Hours
-
Refrigerated (2-8°C):
8 Days
Store
Transport (Cold Packs)
-
Frozen (-20° C or colder):
30 Days
Store
-OR-
0.3 mL Eye Fluid in Plastic Vial
Minimum Volume:
0.25 mL Eye Fluid** This volume does not allow for repeat testing
Collection Instructions:
Collect in a sterile, leak proof, plastic container
Processing Instructions:
Do not dilute collected specimen with additional saline or other transport media
Transport and Storage:
-
Ambient (18-24°C):
48 Hours
Store
-
Refrigerated (2-8°C):
8 Days
Store
Transport (Cold Packs)
-OR-
1.0 mL Urine in a Yellow Capped Conical Tube
Minimum Volume:
0.3 mL Urine** This volume does not allow for repeat testing
Specimen Information:
Collection Instructions:
Clean Catch Midstream Collection
Patient Collection Instructions
Pediatric Collection Instructions
Transport and Storage:
-
Ambient (18-24°C):
48 Hours
Store
-
Refrigerated (2-8°C):
8 Days
Store
Transport (Cold Packs)
-
Frozen (-20° C or colder):
30 Days
Store
Clinical Interpretation
Reference Range:
Not Detected
Methodology:
- Real-Time Polymerase Chain Reaction
Clinical Significance
CMV infections are common and usually asymptomatic. In patients who are immunocompromised, CMV may cause
disseminated, severe disease. CMV may cause birth defects in a minority of infected newborns. DNA methods provide the highest sensitivity and specificity of any method.
Production Schedule
Sites Performed
- Quest - Chantilly
Days Performed
Sunday
Monday
Tuesday
Wednesday
Thursday
Friday
Saturday
Departments
- Sendouts - Clinical
Turn Around Time
2 to 6 days
Coding & Compliance
CDM
00911345
CPT Coding
87496