Histoplasma Antigen, Quantitative

Overview

  • EPIC Code:
  • LAB2897
  • Soft Test Code:
  • SHISG
  • Send Out Test Code:
  • 310
Alternate Names
  • Histoplasma Ag
  • Histoplasma Ag Quantitative EIA
  • Histoplasma Antigen
  • Histoplasma Quantitative EIA Test
  • MVista® Histoplasma Ag Quantitative EIA
Clinical Significance

The MVista® histoplasma quantitative antigen test aids the diagnosis of histoplasmosis. Monitoring the histoplasmosis helps determine when treatment can be stopped and to diagnose relapse.

The result is not intended to be used as the sole means for the clinical diagnosis or patient management decisions.

  • Cross-reactions are seen with blastomycosis, paracoccidioidomycosis, penicilliosis, less frequently in coccidioidomycosis, rarely in aspergillosis and possibly sporotrichosis.
  • Sputolysin, sodium hydroxide and potassium hydroxide treatment degrade the analyte detected in the assay.


Specimen Collection & Preparation

Specimen Requirements:

-OR-

2.0 mL Serum from a SST Gold Top Tube 
Minimum Volume:
1.2 mL Serum*

* This volume does not allow for repeat testing

Processing Instructions:

Allow blood to clot for 30 minutes

Centrifuge and separate from cells 


Rejection Criteria:

If specimen is too viscous to pipette

Stored in Transport Media, Fixative or Isolator Tubes

Tissue, Sputum, Bronchial Brushings, Stool, FNA, Biopsy, Tracheal or Bone Marrow Aspirate


Transport and Storage:
  • Ambient: 2 Weeks
  • Refrigerated: 2 Weeks
  • Frozen (-20 C or colder): Indefinitely

-OR-

2.0 mL Plasma from a Lavender Top Tube - EDTA in a Plastic Vial
Minimum Volume:
1.2 mL Plasma*

* This volume does not allow for repeat testing

Processing Instructions:

Centrifuge specimen for 15 minutes and pipette into a plastic vial

Identify specimen as plasma on container


Rejection Criteria:

If specimen is too viscous to pipette

Stored in Transport Media, Fixative or Isolator Tubes

Tissue, Sputum, Bronchial Brushings, Stool, FNA, Biopsy, Tracheal or Bone Marrow Aspirate


Transport and Storage:
  • Ambient: 2 Weeks
  • Refrigerated: 2 Weeks
  • Frozen (-20 C or colder): Indefinitely

Clinical Interpretation

Reference Range:

Non Detected


Test Comments:

Positive: 0.20-20.00 ng/mL

Positive Above the Limit of Quantification: Results greater than 20.00 ng/mL fall outside the linear range of the assay. These results are positive, but not accurately quantifiable.


Methodology:
  • Quantitative Sandwich Enzyme Immunoassay (EIA)
  • Serum and CSF are pre-treated to improve sensativity
Clinical Significance

The MVista® histoplasma quantitative antigen test aids the diagnosis of histoplasmosis. Monitoring the histoplasmosis helps determine when treatment can be stopped and to diagnose relapse.

The result is not intended to be used as the sole means for the clinical diagnosis or patient management decisions.

  • Cross-reactions are seen with blastomycosis, paracoccidioidomycosis, penicilliosis, less frequently in coccidioidomycosis, rarely in aspergillosis and possibly sporotrichosis.
  • Sputolysin, sodium hydroxide and potassium hydroxide treatment degrade the analyte detected in the assay.


Documentation

*New positives may require confirmation 


Production Schedule

Sites Performed
  • MiraVista
Days Performed
Monday
Tuesday
Wednesday
Thursday
Friday
Departments
  • Sendouts - Clinical
Turn Around Time

1 to 2 days


Coding & Compliance

CDM

00917385


CPT Coding

87385