Bleeding Time

Overview

  • EPIC Code:
  • LAB333
  • Soft Test Code:
  • BLED
Alternate Names
  • PFA 100
  • Platelet Function Assay 100
Included Tests

Collagen EPI; Collagen ADP


Clinical Significance

Coll/EPI          Coll/ADP
<=173 sec       Not Done
Platelet Function appears adequate. The effects of ADP receptor blockers such as Clopidogrel are unpredictable.

Coll/EPI           Coll/ADP

>173 sec          <=126 sec
An Elevated Collagen/EPI result with a Normal/Low Collagen/ADP result is consistent with any of the following: mild thrombocytopenia, low hematocrit, mild platelet dysfunction, mild von Willebrand disease or may be related to recent antiplatelet medication including aspirin. The effects of ADP receptor blockers such as Clopidogrel are unpredictable.
Coll/EPI            Coll/ADP
>173 sec            >126 sec
An Elevated Collagen/EPI with an Elevated Collagen/ADP result is consistent with any of the following or combination of: thrombocytopenia, low hematocrit, platelet dysfunction, von Willebrand disease or recent antiplatelet medication such as GPllb/llla receptor inhibitors. The effects of ADP receptor blockers such as Clopidogrel are unpredictable. Additional follow-up may be warranted if clinically indicated. 


Specimen Collection & Preparation

Client Notes

Prior to collection, please contact PHL Client Response at 266-1500 (Option 2) to request a Stat courier to maintain the integrity and stability of the specimen. 

If specimen cannot get to Parkview Regional Medical Center (PRMC) within 3 hours of collection, please send the patient to PRMC to be drawn at 11109 Parkview Plaza, Entrance 3. 


Specimen Requirements:

-OR-

5.4 mL Whole Blood in two (2) Blue Top Tubes - 3.2% Na Citrate
Collection Instructions:

Collect using a 21G or larger (20G or 19G) needle.

Collection tubes must be filled to the recommended fill line as specified by the tube manufacturer.


Rejection Criteria:

Specimens that have been centrifuged MUST be recollected.

Specimens that have been refrigerated, chilled on ice, or frozen MUST be recollected.

Specimens must be tested within 4 hours of collection- recollect if stability time is exceeded.

Hemolyzed or clotted samples are unacceptable.


Transport and Storage:
  • Ambient (18-24°C): 4 Hours

    Specimens must be received at testing site within 3 hours of collection.

Clinical Interpretation

Reference Range:

Collagen/EPI: 69 - 173 seconds 
Collagen/ADP: 54 - 126 seconds


Test Comments:

Collagen/EPI will always be performed. If Collagen/EPI is > 173 seconds, Collagen/ADP testing will be performed.


Methodology:
  • Platelet Function Analyzer
Clinical Significance

Coll/EPI          Coll/ADP
<=173 sec       Not Done
Platelet Function appears adequate. The effects of ADP receptor blockers such as Clopidogrel are unpredictable.

Coll/EPI           Coll/ADP

>173 sec          <=126 sec
An Elevated Collagen/EPI result with a Normal/Low Collagen/ADP result is consistent with any of the following: mild thrombocytopenia, low hematocrit, mild platelet dysfunction, mild von Willebrand disease or may be related to recent antiplatelet medication including aspirin. The effects of ADP receptor blockers such as Clopidogrel are unpredictable.
Coll/EPI            Coll/ADP
>173 sec            >126 sec
An Elevated Collagen/EPI with an Elevated Collagen/ADP result is consistent with any of the following or combination of: thrombocytopenia, low hematocrit, platelet dysfunction, von Willebrand disease or recent antiplatelet medication such as GPllb/llla receptor inhibitors. The effects of ADP receptor blockers such as Clopidogrel are unpredictable. Additional follow-up may be warranted if clinically indicated. 


Production Schedule

Sites Performed
  • Parkview Regional Medical Center
Days Performed
Sunday
Monday
Tuesday
Wednesday
Thursday
Friday
Saturday
Departments
  • Coagulation
Turn Around Time

4 hours


Stat Eligible

Coding & Compliance

CDM

01455578 (Reflex 01455579)


CPT Coding

85576 (Reflex 85576)