Centrifuge light blue-top tube 15 minutes at approximately 1500 g within 60 minutes of collection. Using a plastic pipette, remove plasma, taking care to avoid the WBC/platelet buffy layer and place into a plastic vial. Centrifuge a second time and transfer platelet-poor plasma into four new plastic vials. Plasma must be free of platelets (<10,000/mcL).
2 separate plastic vials 2.0 mL plasma frozen and sent