Medical Necessity Documentation:
Client Notes:
Patient Preparation:
Specimen Requirements:
1.0 mL Serum from a Red Top Tube in a Plastic Vial
Collection Instructions:
Minimum Volume:
0.5 mL Serum
Transport & Storage: Temperature/Stability:
7 days Ambient
14 days Refrigerated
30 days Frozen
Rejection Criteria:
Gross hemolysis • Grossly lipemic
Reference Range:
| ANCA Screen |
Negative |
| C-ANCA Titer |
<1:20 |
| P-ANCA Titer |
<1:20 |
| Atypical P-ANCA Titer |
<1:20 |
Critical Ranges:
Test Comments:
If ANCA Screen is positive, then C-ANCA Titer and/or P-ANCA Titer, and/or atypical P-ANCA Titer will be performed at an additional charge (CPT code(s): 86021 for each titer performed)
Methodology:
Immunoassay (IA)
Clinical Significance:
Testing for anti-neutrophil cytoplasmic antibodies (P-ANCA and or C-ANCA) has been found to be useful in establishing the diagnosis of suspected vascular diseases (e.g., crescentic glomerulonephritis, microscopic polyarteritis and churg-strauss syndrome), bowel disease (Crohn's Disease, ulcerative colitis, primary sclerosing cholangitis, and autoimmune hepatitis) as well as with other autoimmune diseases (drug-induced lupus, SLE, Felty's Syndrome).
ANCA has classically been divided into C-ANCA and P-ANCA depending on the immunofluorescent pattern observed. More recently the specific antigens responsible for these patterns have been described and isolated. The antigen that gives the C-ANCA pattern is Proteinase-3 (PR-3). Multiple antigens are responsible for P-ANCA pattern, the principle antigen being Myeloperoxidase (MPO).
Patients with bowel disease have been shown to have antibodies that give a P-ANCA or C-ANCA pattern. These antibodies however may not be directed towards MPO. Patients with drug induced lupus, etc, often present with a P-ANCA pattern that is associated with antibodies against MPO.
Documentation:
Custom Panel:
No
PRODUCTION SCHEDULE
Turn Around Time:
3 to 5 days
Days Performed:
Monday, Tuesday, Wednesday, Thursday, Friday, Saturday
Sites Performed:
Quest - Chantilly
PHL Test Code:
ANCAT
EPIC Test Code:
IMO5092
Send Out Test Code:
70171
Alternate Test Names:
ANCA Scrn, Reflex ANCA Titer
Included Tests:
ANCA Screen; Reflex (Atypical P-ANCA Titer; C-ANCA Titer; P-ANCA Titer)
CPT Coding:
86036, 86037 x3