ANCA Screen with Reflex to ANCA Titers

Last Modified: 9/19/2019 1:28:14 PM


Specimen Requirements: 1.0 mL Serum from a Red Top Tube in a Plastic Vial
Minimum Volume: 0.5 mL Serum
Transport & Storage: Temperature/Stability: 7 days Ambient
14 days Refrigerated
30 days Frozen
Rejection Criteria: Gross hemolysis • Grossly lipemic
Reference Range:
ANCA Screen Negative
  C-ANCA Titer <1:20
  P-ANCA Titer <1:20
  Atypical P-ANCA Titer <1:20
Test Comments: If ANCA Screen is positive, then C-ANCA Titer and/or P-ANCA Titer, and/or atypical P-ANCA Titer will be performed at an additional charge (CPT code(s): 86021 for each titer performed)
Methodology: Immunoassay (IA)
Clinical Significance: Testing for anti-neutrophil cytoplasmic antibodies (P-ANCA and or C-ANCA) has been found to be useful in establishing the diagnosis of suspected vascular diseases (e.g., crescentic glomerulonephritis, microscopic polyarteritis and churg-strauss syndrome), bowel disease (Crohn's Disease, ulcerative colitis, primary sclerosing cholangitis, and autoimmune hepatitis) as well as with other autoimmune diseases (drug-induced lupus, SLE, Felty's Syndrome).
ANCA has classically been divided into C-ANCA and P-ANCA depending on the immunofluorescent pattern observed. More recently the specific antigens responsible for these patterns have been described and isolated. The antigen that gives the C-ANCA pattern is Proteinase-3 (PR-3). Multiple antigens are responsible for P-ANCA pattern, the principle antigen being Myeloperoxidase (MPO).
Patients with bowel disease have been shown to have antibodies that give a P-ANCA or C-ANCA pattern. These antibodies however may not be directed towards MPO. Patients with drug induced lupus, etc, often present with a P-ANCA pattern that is associated with antibodies against MPO.
Custom Panel: No

PRODUCTION SCHEDULE

Turn Around Time: 3 to 5 days
Days Performed: Monday, Tuesday, Wednesday, Thursday, Friday, Saturday
Sites Performed: Quest - Chantilly
PHL Test Code: ANCAT
Send Out Test Code: 70171
Included Tests: ANCA Screen; Reflex (Atypical P-ANCA Titer; C-ANCA Titer; P-ANCA Titer)
CPT Coding: 86021 x 4

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