Chlamydia trachomatis Culture

Last Modified: 8/13/2020 7:26:34 AM

Medical Necessity Documentation:  
Client Notes: This specimen must reach the Parkview Health Laboratories within 48 hours of collection, please contact the client response department to verify the possible reciept time of the specimen, and the possibility of a special courier.
Patient Preparation:  
Specimen Requirements: V-C-M Media (Green Top)

Endocervical, Urethral, throat, and Nasal/Nasopharyngeal Swabs
Rectal mucosa swab (without feces)
Fresh (unfixed) Tissue
3.0 mL pelvic washings
Fresh Biopsy
Fluids: Pleural, Peritoneal, Body
Pelvic Washings
Collection Instructions:

Order must include the site and/or source of collection

This media comes in site specific collection kits for Cervical, Lesion / Other, Nasopharyngeal, Urethral

Break or bend swabs to fit inside V-C-M Media (Green Top)

Immerse Fresh Biopsy in the V-C-M Media (Green Top).

After an aseptic collection of Pelvic Washings or Fluids: Pleural, Beritoneal, Body; transfer 3 mL in the V-C-M Media (Green Top)in equal volume (i.e., 3 mL fluid + 3 mL V-C-M Media)

Nasal/Nasopharyngeal Collection Instructions

Minimum Volume: 3.0 mL
Transport & Storage: Temperature/Stability: Can be stored Refrigerated for up to 48 hours,

DO NOT Transport Refrigerated,.

Specimen must be Frozen at -70 degree, in the Quick Freeze -70 Freezer at PRMC or on Dry Ice within 48 hours of collection.

Once Frozen Stable 30 days

It is unacceptable to freeze at -20 degrees (Regular Refrigerator Freezer)
Rejection Criteria: Viral Transport Media that does not support Chlamydia, wooden shaft and calcium alginate swabs, dry swabs, transwabs or swabs in bacterial gel-based, DNA Probe, in formalin/fixatives, transports for antigen detection by EIA; eye conjunctival specimen
Reference Range: No Chlamydia Isolated
Critical Ranges:  
Test Comments:  
Methodology: Centrifugation-Enhanced Culture with Monoclonal Antibody Detection
Clinical Significance: C. trachomatis is associated with infections of the mucous membranes of the urogenital system, the upper respiratory tract and the eye. It may be sexually transmitted; resulting diseases include urethritis, cervicitis, salpingitis, epididymitis, proctitis and lymphogranuloma venereum. 
C. pneumoniae is associated with both upper and lower respiratory infections. Infections of the upper respiratory tract and eye usually occur in newborns exposed at parturition. In adults, eye infection may be transmitted by hand after contact with secretions. Isolation by tissue culture is recommended when testing individuals for legal purposes.
Documentation:  Note: Due to potential propagation of SARS-CoV-2 virus in Chlamydia culture cells, respiratory and eye specimens are no longer accepted. Teh recommended alterative for respiratory and eye specimens is Chlamydia tachomatis Antigent DFA available at Quest 
Custom Panel: No


Turn Around Time: 3 days
Days Performed: Monday, Tuesday, Wednesday, Thursday, Friday, Saturday
Sites Performed: Quest - Chantilly
PHL Test Code: CHLM
EPIC Test Code: LAB244
Send Out Test Code: 877
Alternate Test Names: Also ordered for Chlamydia Culture on the Eye; Chlamydia Culture; Chlamydia trachomatis Culture; Culture, Chlamydia trachomatis
Included Tests:  
CPT Coding: 87110, 87140

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