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ACTH Stimulation Test

Last Modified: 3/25/2009 6:41:56 PM


Client Notes:

ACTH Stimulation Testing should be scheduled in advance with Enterprise Scheduling 373-8900
Specimen Requirements: 1.0 mL Serum from a Red Top Tube in a Plastic Vial - One for each timed draw
Collection Instructions:
  1. Request a base line level Cortisol.
  2. After this is drawn, the patient is to be given a 250-uG dose of synthetic ACTH, Cortrosyn, IM.
  3. Request a second Cortisol to be drawn one-half hour after this dose is given.
  4. Request a third Cortisol to be drawn one hour after the dose is given

Example: 
1. Base Line Cortisol - 8:00 A.M.
2. ACTH Dose - 8:05 A.M. 
3. One-Half Hour Cortisol - 8:35 A.M 
4. One Hour Cortisol - 9:05 A.M.
Minimum Volume: 0.5 mL
Temperature & Stability: 48 hours Refrigerated
Reference Range:
No reference range established
Test Comments:
A normal increase of at least 7 UG/DL of Cortisol is expected for a short ACTH stimulation test.
Methodology: Chemiluminscence
Clinical Significance: Method for testing the adrenal reserve to rule out Adison Disease

PRODUCTION SCHEDULE

Days Performed: Monday, Tuesday, Wednesday, Thursday, Friday, Saturday
Sites Performed: Parkview Central Laboratory
PHL Test Code: ACTHS
CPT Coding: 82533 x 3

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