Fungus Culture, Blood

Last Modified: 9/21/2021 10:13:29 AM

Medical Necessity Documentation:

Effective Immediately - Due to a supply shortage, Adult Fungal Blood Culture Tubes Parkview will be temporarily sending to Mayo Clinic.

Client Notes: Please contact PHL Client Response at 266-1500 (Option 1) to request special collection tubes and pick up.

Transport immediately to the laboratory after collection

Patient Preparation:  
Specimen Requirements: 10.0 mL Whole Blood in a Wampole Isolator Microbial Tube  Example

Pediatric: 1.5 mL Whole Blood in a Pediatric Wampole Isolator Microbial Tube Example 

MAYO Clinic Calls for 10.0-30.0 mL (5.0mL) Whole Blood in a Green Top Tube Na Heparin
Collection Instructions:

Speciman must be to PRMC within 12 hours of collection. Contact Client Response @ 266-1500 Option 1 if Stat Courier is needed.

Please indicate any prior antibiotic therapy

  1. Wash and dry hands thoroughly or use an approved hand antiseptic and observe Universal Precautions.
  2. Sterilize the stopper on the isolator tube with iodine. Do not allow it to pool on the stopper as it could enter the tube and kill organisms in the sample.
  3. Allow disinfectant to dry completely.
  4. Always collect the isolator specimens before collecting other specimens in order to avoid contamination of the fungal blood culture.
  5. Apply tourniquet and select a site for the venipuncture. The tourniquet should not be left on for more than 1 minute as this causes hemoconcentration, which can affect lab results.
  6. Prepare site with alcohol using outward concentric circles; let dry.
  7. Prepare site with iodine using outward concentric circles; let air dry for 1-2 minutes.
  8. Assemble needle into Needle-Pro Safety Device.
  9. ADD: If palpation is required after skin disinfection, sterilize the palpating finger with iodine. This minimizes cross-contamination of a sterile site.
  10. Collect the blood with the patient’s arm in a downward position. Hold the tube/needle assembly so that the tube bottom is lower than the needle.
  11.  Allow the tube to fill completely. Flow will stop when the tube is full. Short samples decrease the chance of isolating already low numbers of organisms. 
  12. Gently invert the tube 5-10 times to completely mix blood with the isolator tube additive. 
  13. Clotted specimens will be rejected.
  14. Label blood culture bottle(s) with patient's full legal name, DOB, date and time, site of draw.

 BioMeriux Videos to aid in the collection of Blood Cultures: 

Minimum Volume: 7.0 mL Whole Blood - Pediatric 0.5 mL Whole Blood
Transport & Storage: Temperature/Stability: 16 hours Ambient

Do not refrigerate or incubate the specimen as recovery of organisms may be dramatically decreased

This specimen must be processed by the laboratory within 16 hours of collection
Reference Range: No Growth
Critical Ranges:  
Test Comments:
Specimens are inoculated onto artificial growth media and incubated sufficient time to allow growth of the suspected microbe. Media type, incubation conditions and period of incubation may vary with the type of microorganism suspected.

Appropriate identification and determination of organism susceptibility is available for yeast isolates upon request. Susceptibility testing will be sent to Quest Laboratory to be performed.
Preliminary negative results are reported weekly
Methodology: Isostat Microbial System
Clinical Significance:  
Custom Panel: No


Turn Around Time: Positive Immediately / Final Negative 4 weeks
Days Performed: Sunday, Monday, Tuesday, Wednesday, Thursday, Friday, Saturday
Sites Performed: Parkview Regional Medical Center
PHL Test Code: CXBFU
EPIC Test Code: LAB242
Alternate Test Names: Culture, Fungus Blood; Fungal Blood Culture
Included Tests:  
CPT Coding: 87015; 87103 (Reflexes 87106, 87107)

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