Herpes Simplex Virus, Type 1 and 2 DNA, Quantitative

Overview

  • EPIC Code:
  • LAB3146
  • Soft Test Code:
  • Send Out Test Code:
  • 19502
Alternate Names
  • Herpes Simplex Virus DNA UltraRapid, CSF
  • Herpes Simplex Virus, Type 1 and 2 DNA, Quantitative, Real-Time PCR
  • HSV DNA Ultra Rapid CSF
  • HSV, 1 AND 2 DNA, QUANT PCR
  • HSVQN
Included Tests

Herpes Simplex Virus (HSV) 1 DNA; Herpes Simplex Virus (HSV) 2 DNA


Clinical Significance

The detection of HSV-1 and HSV-2 DNA is based upon the real-time amplification, detection and differentiation of specific HSV-1 and HSV-2 genomic DNA sequences by PCR from total DNA extracted from the specimen. The quantitative range of this assay is 100-2,000,000 copies/mL.


Specimen Collection & Preparation

Specimen Overview

Specimen type must be written on the tube

Specimen Requirements:

-OR-

1.0 mL CSF in a Sterile Plastic Container
Minimum Volume:
0.3 mL CSF*

* This volume does not allow for repeat testing

Rejection Criteria:

Specimen frozen in a glass tube  


Transport and Storage:
  • Ambient (18-24°C): 48 Hours
  • Refrigerated (2-8°C): 7 Days
  • Frozen (-20° C or colder): 30 Days

    Transport 

-OR-

1.0 mL Vitreous in a Sterile Plastic Container
Minimum Volume:
0.3 mL Vitreous*

* This volume does not allow for repeat testing

Rejection Criteria:

Specimen frozen in a glass tube  


Transport and Storage:
  • Ambient (18-24°C): 48 Hours
  • Refrigerated (2-8°C): 7 Days
  • Frozen (-20° C or colder): 30 Days

    Transport 

-OR-

1.0 mL Pericardial in a Sterile Plastic Container
Minimum Volume:
0.3 mL Vitreous*

* This volume does not allow for repeat testing

Rejection Criteria:

Specimen frozen in a glass tube  


Transport and Storage:
  • Ambient (18-24°C): 48 Hours
  • Refrigerated (2-8°C): 7 Days
  • Frozen (-20° C or colder): 30 Days

    Transport 

-OR-

1.0 mL Pleural Fluid a Sterile Plastic Container
Minimum Volume:
0.3 mL Pleural Fluid*

* This volume does not allow for repeat testing

Rejection Criteria:

Specimen frozen in a glass tube  


Transport and Storage:
  • Ambient (18-24°C): 48 Hours
  • Refrigerated (2-8°C): 7 Days
  • Frozen (-20° C or colder): 30 Days

    Transport

-OR-

1.0 mL Amniotic Fluid a Sterile Plastic Container
Minimum Volume:
0.3 mL Amniotic Fluid*

* This volume does not allow for repeat testing

Rejection Criteria:

Specimen frozen in a glass tube  


Transport and Storage:
  • Ambient (18-24°C): 48 Hours
  • Refrigerated (2-8°C): 7 Days
  • Frozen (-20° C or colder): 30 Days

    Transport 

-OR-

1.0 mL Plasma from a Lavender Top Tube - EDTA in a Plastic Vial
Minimum Volume:
0.3 mL Plasma*

* This volume does not allow for repeat testing

Rejection Criteria:

Specimen frozen in a glass tube  


Transport and Storage:
  • Ambient (18-24°C): 48 Hours
  • Refrigerated (2-8°C): 7 Days
  • Frozen (-20° C or colder): 30 Days

    Transport 

-OR-

1.0 mL Plasma from a Yellow Top Tube - ACD in a Plastic Vial
Minimum Volume:
0.3 mL Plasma*

* This volume does not allow for repeat testing

Rejection Criteria:

Specimen frozen in a glass tube  


Transport and Storage:
  • Ambient (18-24°C): 48 Hours
  • Refrigerated (2-8°C): 7 Days
  • Frozen (-20° C or colder): 30 Days

    Transport

-OR-

1.0 mL Whole Blood in a Lavender Top Tube - EDTA
Minimum Volume:
0.3 mL Whole Blood*

* This volume does not allow for repeat testing

Rejection Criteria:

Specimen frozen 


Transport and Storage:
  • Ambient (18-24°C): 48 Hours
  • Refrigerated (2-8°C): 7 Days

    Transport

    Cold Packs

  • Frozen (-20° C or colder): Unacceptable

-OR-

1.0 mL Whole Blood in a Yellow Top Tube - ACD
Minimum Volume:
0.3 mL Whole Blood*

* This volume does not allow for repeat testing

Rejection Criteria:

Specimen frozen 


Transport and Storage:
  • Ambient (18-24°C): 48 Hours
  • Refrigerated (2-8°C): 7 Days

    Transport

    Cold Packs

  • Frozen (-20° C or colder): Unacceptable

-OR-

1.0 mL Serum from a Red Top Tube in a Plastic Vial
Minimum Volume:
0.3 mL Serum*

* This volume does not allow for repeat testing

Rejection Criteria:

Specimen frozen in a glass tube  


Transport and Storage:
  • Ambient (18-24°C): 48 Hours
  • Refrigerated (2-8°C): 7 Days
  • Frozen (-20° C or colder): 30 Days

     Transport

Clinical Interpretation

Reference Range:

HSV 1, DNA, QN, PCR Not Detected
HSV 2, DNA, QN, PCR Not Detected


Methodology:
  • Real-Time Polymerase Chain Reaction
Clinical Significance

The detection of HSV-1 and HSV-2 DNA is based upon the real-time amplification, detection and differentiation of specific HSV-1 and HSV-2 genomic DNA sequences by PCR from total DNA extracted from the specimen. The quantitative range of this assay is 100-2,000,000 copies/mL.


Production Schedule

Sites Performed
  • Quest - Chantilly
Days Performed
Sunday
Monday
Tuesday
Wednesday
Thursday
Friday
Saturday
Departments
  • Sendouts - Clinical
Turn Around Time

1 to 2 days


Coding & Compliance

CDM

00917077, 00917078


CPT Coding

87530 x 2