Acute Leukemia Panel, Flow Cytometry

Overview

  • EPIC Code:
  • LAB18303
  • Soft Test Code:
Alternate Names
  • Acute Leukemia Panel
  • FACUT
  • Flow Cytometry, Acute
Included Tests

CBC W/ AUTO DIFFERENTIAL [White Blood Cell Count (WBC); Red Blood Cell Count (RBC); Hemoglobin (Hgb); Hematocrit (Hct); Mean Corpuscular Volume (MCV); Mean Corpuscular Hemoglobin (MCH); Mean Corpuscular Hemoglobin Concentration (MCHC); Red Cell Distribution Width Standard Deviation (RDWSD); Red Cell Distribution Width Coefficient Variation (RDWCV); Platelet (PLT); Mean Platelet Volume (MPV); Nucleated Red Blood Cell (nRBC); Differential automated, reflexes to manual if indicated]; CD2; CD3; CD4; CD5; CD7; CD8; CD10; CD11b; CD13; CD14; CD15; CD16; CD19; CD20; CD22; CD23; CD33; CD34; CD36; CD38; CD41; CD45; CD56; CD57; CD64; CD71; CD79b; CD117; HLA-DR; Surface Kappa; Surface Lambda

 


Included Tests References:
Clinical Significance

Useful for distinguishing acute lymphoblastic leukemia from acute myelogenous leukemia and phenotypic subtyping of acute leukemias


Specimen Collection & Preparation

Client Notes

Complete a requisition order, with orders for Acute Leukemia Panel, Flow cytometry [LAB18303], CBCWD [LAB293], and a Hold for Flow Lavender [LAB18016].

  • Draw one SODIUM HEPARIN-Green top tube for the Flow Cytometry panel.
  • Draw two EDTA-Lavender top tubes on will be for the CBCWD and the other will be used for Flow Cytometry panel.

A copy of the requisition must be sent with the samples to the Flow Cytometry Department.


Specimen Requirements:

-AND-

4.0 Whole Blood in a Sodium Heparin Green Top Tube
Minimum Volume:
0.5 mL Whole Blood*

* This volume does not allow for repeat testing

Processing Instructions:

Do not centrifuge


Rejection Criteria:

Refrigerated specimen

Centrifuged specimen

>48 hours 

Lithium heparin green top tube


Transport and Storage:
  • Ambient (18-24°C): 48 Hours

-AND-

3.0 mL Whole Blood in a Lavender Top Tube - EDTA (Lavender top tube)
Minimum Volume:
0.5 mL Whole Blood *

* This volume does not allow for repeat testing

Specimen Information:

EDTA tube is for the Hold for Flow, this is required to be sent with the Sodium Heparin tube.


Processing Instructions:

Do not centrifuge


Rejection Criteria:

Refrigerated specimen

Centrifuged specimen

> 48 hours 


Transport and Storage:
  • Ambient (18-24°C): 48 Hours

Clinical Interpretation

Reference Range:

Refer to Interpretive Pathology Flow Report

See CBC for its Reference Ranges


Test Comments:

The specimen is processed by whole blood lysis and stained with monoclonal antibodies using a direct immunofluorescence method. The cells are separated based on light scatter characteristics. and CD45 staining characteristics.

A Complete Blood Count with Differential will be reflexed, and charged with the ordering of this test if indicated.


Methodology:
  • Flow Cytometry (FC)
Clinical Significance

Useful for distinguishing acute lymphoblastic leukemia from acute myelogenous leukemia and phenotypic subtyping of acute leukemias


Documentation

This test was developed and its performance characteristics determined by Parkview Health Laboratories. It has not been cleared or approved by the FDA. The laboratory is regulated under CLIA as qualified to perform high-complexity testing. This test is used for clinical purposes. It should not be regarded as investigational or for research.


Production Schedule

Sites Performed
  • Parkview Regional Medical Center
Days Performed
Monday
Tuesday
Wednesday
Thursday
Friday
Saturday
Departments
  • Flow Cytometry
Turn Around Time

48 to 72 hours


Coding & Compliance

CDM

01399703, 01399705, 01430747


CPT Coding

85025, 88184, 88185 x 30 (Reflex 85007, 85008)