ADAMTS13 Activity with Reflex to Inhibitor

Last Modified: 8/25/2021 1:35:22 PM


Medical Necessity Documentation:  
Client Notes:  
Patient Preparation:  
Specimen Requirements: 1.0 mL Frozen Platelet Poor Plasma from a Blue Top Tube - 3.2% Na Citrate in a Plastic Vial
Collection Instructions: Please submit a separate, frozen vial for each special coagulation assay ordered. 

Platelet-poor plasma:  Draw blood in a light blue-top tube containing 3.2% sodium citrate, mix gently by inverting 3-4 times. Centrifuge 15 minutes at 1500 g within one hour of collection. Using a plastic pipette, remove plasma, taking care to avoid the WBC/platelet buffy layer and place into a plastic vial. 

Freeze immediately and ship on dry ice.

Please submit a separate, frozen vial for each special coagulation assay ordered.
Minimum Volume: 0.5 mL Frozen Platelet Poor Plasma
Transport & Storage: Temperature/Stability: Unacceptable Ambient
Unacceptable Refrigerated

21 days Frozen -20° (Transport)
Rejection Criteria: Gross hemolysis • Grossly lipemic • Grossly icteric
Reference Range:
ADAMTS13 Activity 68-163 % Activity
ADAMTS13 Inhibitor <0.4 BEU
Critical Ranges:  
Test Comments: If ADAMTS13 Activity is ≤30, then ADAMTS13 Inhibitor will be performed at an additional charge (CPT code(s): 85335)
Methodology: Immunoassay (IA)
Clinical Significance: ADAMTS13 Activity with Reflex to Inhibitor [14532X] - ADAMTS-13 is a zinc metalloprotease that cleaves ultra large vWF multimers. Studies have shown that low levels of ADAMTS-13 activity are associated with thrombotic thrombocytopenic purpura (TTP), a life-threatening hematological condition characterized by a low platelet count, microvascular thrombi, red cell fragmentation, and renal complications. Congenital TTP is a rare inherited disease caused by mutations within the ADAMTS-13 gene, which result in the production of non-functional protein. The acquired form of TTP is an autoimmune-like disorder caused by the development of autoantibodies to ADAMTS-13 that inhibits enzyme activity
Documentation:  
Custom Panel: No

PRODUCTION SCHEDULE

Turn Around Time: 2 to 5 days
Days Performed: Sunday, Monday, Tuesday, Wednesday, Thursday, Friday
Sites Performed: Quest - Chantilly
PHL Test Code: ATS13
EPIC Test Code: LAB14498
Send Out Test Code: 145328
Alternate Test Names: ADAMTS13 Evaluation; von Willebrand Factor Protease Activity (ADAMTS-13; VWF Cleaving Protease (vWF-CP); VWF Cleaving Protease (vWF-CP)(ADAMTS-13); vWF Protease Activity (ADAMTS-13)
Included Tests:  
CPT Coding: 85397

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