Medical Necessity Documentation:
Client Notes:
Patient Preparation:
Specimen Requirements:
1.0 mL Frozen Platelet Poor Plasma from a Blue Top Tube - 3.2% Na Citrate in a Plastic Vial
Collection Instructions:
Please submit a separate, frozen vial for each special coagulation assay ordered.
Platelet-poor plasma: Draw blood in a light blue-top tube containing 3.2% sodium citrate, mix gently by inverting 3-4 times. Centrifuge 15 minutes at 1500 g within one hour of collection. Using a plastic pipette, remove plasma, taking care to avoid the WBC/platelet buffy layer and place into a plastic vial.
Freeze immediately and ship on dry ice.
Please submit a separate, frozen vial for each special coagulation assay ordered.
Minimum Volume:
0.5 mL Frozen Platelet Poor Plasma
Transport & Storage: Temperature/Stability:
Unacceptable Ambient
Unacceptable Refrigerated
21 days Frozen -20° (Transport)
Rejection Criteria:
Gross hemolysis • Grossly lipemic • Grossly icteric
Reference Range:
| ADAMTS13 Activity |
68-163 % Activity |
| ADAMTS13 Inhibitor |
<0.4 BEU |
Critical Ranges:
Test Comments:
If ADAMTS13 Activity is ≤30, then ADAMTS13 Inhibitor will be performed at an additional charge (CPT code(s): 85335)
Methodology:
Immunoassay (IA)
Clinical Significance:
ADAMTS13 Activity with Reflex to Inhibitor [14532X] - ADAMTS-13 is a zinc metalloprotease that cleaves ultra large vWF multimers. Studies have shown that low levels of ADAMTS-13 activity are associated with thrombotic thrombocytopenic purpura (TTP), a life-threatening hematological condition characterized by a low platelet count, microvascular thrombi, red cell fragmentation, and renal complications. Congenital TTP is a rare inherited disease caused by mutations within the ADAMTS-13 gene, which result in the production of non-functional protein. The acquired form of TTP is an autoimmune-like disorder caused by the development of autoantibodies to ADAMTS-13 that inhibits enzyme activity
Documentation:
Custom Panel:
No
PRODUCTION SCHEDULE
Turn Around Time:
2 to 5 days
Days Performed:
Sunday, Monday, Tuesday, Wednesday, Thursday, Friday
Sites Performed:
Quest - Chantilly
PHL Test Code:
ATS13
EPIC Test Code:
LAB14498
Send Out Test Code:
145328
Alternate Test Names:
ADAMTS13 Evaluation; von Willebrand Factor Protease Activity (ADAMTS-13; VWF Cleaving Protease (vWF-CP); VWF Cleaving Protease (vWF-CP)(ADAMTS-13); vWF Protease Activity (ADAMTS-13)
Included Tests:
CPT Coding:
85397