Body Fluid/Tissue Lymphoma Panel, Flow Cytometry

Overview

  • EPIC Code:
  • LAB6946
  • Soft Test Code:
Alternate Names
  • Body Fluid, Flow Cytometry
  • CSF Flow Cytometry
  • FLFLO
  • Flow Cytometry Lymphoma
  • Tissue, Flow Cytometry
Included Tests

CD2; CD3; CD4; CD5; CD7; CD8; CD10; CD16; CD19; CD20; CD22; CD23; CD38; CD45; CD56; CD57; CD79b; CD200; FMC7; Surface Kappa; Surface Lambda


Clinical Significance

Immunophenotyping by Flow Cytometry is used on fresh tissue biopsies to distinguish lymphomas from reactive lymph nodes and in the sub-classification of non-Hodgkin's lymphomas.

Body fluids can be used to substantiate the diagnosis, direct treatment, and predict outcome based on the cells present in the fluid.


Specimen Collection & Preparation

Client Notes

This test is for tissues, lymph nodes, and body fluids ONLY. If peripheral blood or bone marrow see B-Cell CLL/Lymphoma Panel.

Place tissues and lymph nodes in RPMI media as soon as possible after collection.

Body fluids should be collected into sterile collection containers. Deliver entire collection container to the laboratory to be aliquotted by laboratory staff.

A copy of the requisition must accompany the sample to the laboratory and the Flow department.


Specimen Overview

Tissue biospy or Fine Needle Aspirates must be placed in RPMI media as soon after collection as possible. Contact the Flow Cytometry Department (260-266-1580) for RPMI tubes if needed. 

Body Fluids are preferred in their original collection container, especially for CSF. Lavender EDTA tube or Sodium Heparin green top tubes are also acceptable.

Specimen Requirements:

-AND-

Sterile Container Conical Screw Cap Tube, Body Fluid
Specimen Information:

CSF collections should be put in the sterile screw top tubes in CSF collection kits. These collection kits contain four tubes with 1-4 printed on the tube. Flow testing will be performed on tube 3 or 4. 

All other body fluids should be delivered to the laboratory in their original collection container. 


Collection Instructions:

Use a sterile collection container to collect as much fluid as possible. 


Processing Instructions:

Laboratory processing staff will aliquot from original collection container to the appropriate sample containers for the testing ordered.

CSF samples should always remain in the CSF Collection tube, do not aliquot.


Rejection Criteria:

Samples are considered irretrievable and every attempt will be made to process the sample. The Pathologist will determine if sample should be rejected if unacceptable collection, processing, or handling occurs.


Transport and Storage:
  • Refrigerated (2-8°C): Up to 48 hours

-AND-

RPMI Media, Tissues and Lymph Nodes
Collection Instructions:

Tissues, Lymph nodes, and Fine Needle Aspirates should be placed in RPMI media as soon after collection as possible. Contact the Flow Cytometry Department (260-266-1580) if RPMI tubes are needed.


Processing Instructions:

Deliver to the lab as soon as possible.


Rejection Criteria:

Samples are considered irretrievable and every attempt will be made to process the sample. The Pathologist will determine if sample should be rejected if unacceptable collection, processing, or handling occurs.


Transport and Storage:
  • Refrigerated (2-8°C): Up to 48 hours

Clinical Interpretation

Reference Range:

Refer to Interpretive Pathology Flow Report.


Test Comments:

The specimen is prepared by a whole blood lysis method and stained with monoclonal antibodies using a direct immunofluorescence method. The cells are separated based on light scatter characteristics and CD45 staining characteristics.


Methodology:
  • Flow Cytometry (FC)
Clinical Significance

Immunophenotyping by Flow Cytometry is used on fresh tissue biopsies to distinguish lymphomas from reactive lymph nodes and in the sub-classification of non-Hodgkin's lymphomas.

Body fluids can be used to substantiate the diagnosis, direct treatment, and predict outcome based on the cells present in the fluid.


Documentation

This test was developed and its performance characteristics determined by Parkview Health Laboratories. It has not been cleared or approved by the FDA. The laboratory is regulated under CLIA as qualified to perform high-complexity testing. This test is used for clinical purposes. It should not be regarded as investigational or for research.


Production Schedule

Sites Performed
  • Parkview Regional Medical Center
Days Performed
Monday
Tuesday
Wednesday
Thursday
Friday
Saturday
Departments
  • Flow Cytometry
Turn Around Time

48 to 72 hours


Coding & Compliance

CDM

01399703, 01399705


CPT Coding

88184, 88185 x19