BK Virus DNA, Quantitative Real-Time PCR, Plasma,Serum

Last Modified: 9/15/2022 5:45:30 AM

Medical Necessity Documentation:  
Client Notes:  
Patient Preparation:  
Specimen Requirements:  0.7 mL plasma collected in an EDTA tube or ACD tube 
0.7 mL serum collected in a Serum Separator tube 
0.7 mL whole blood collected in an EDTA tube or ACD tube
Collection Instructions: Plasma:  collect blood in sterile tubes containing EDTA or in plasma preparation tube.  Collect whole blood at room temperature and separate plasma fom cells within 2 hours of collection.  Transfer plasma to sterile, plastic, screw-cap aliquot tubes.  Freeze and ship frozen.

Serum:  collect blood in sterile tubes with no anticoagulants;  Serum Separator Tubes (SST) are recommended.  Allow blood to clot at room temperature and separate serum within 1 hour of collection.  Transfer serum to sterile, plastic, screw-cap aliquot tubes.  Freeze and ship frozen.

Whole blood:  collect using standard procedures.  Store samples refrigerated following collection.  **DO NOT freeze whole blood.

Minimum Volume: 0.3 mL
Transport & Storage: Temperature/Stability: Plasma or serum:  transport frozen
Whole blood: transport refrigerated

Plasma and serum
Ambient 48 hours
Refrigerated 7 days
Frozen 30 days

Whole blood
Ambient 48 hours
Refrigerated 8 days
Frozen unacceptable

Rejection Criteria: Whole blood in heparin tube
Reference Range:  Not detected
Critical Ranges:  
Test Comments:  
Methodology: Real-Time Polymerase Chain Reaction (RT-PCR)
Clinical Significance: This test is used to determine the presence of BK virus DNA in patients' specimens.  Detection of the virus in these specimens may be indicative of an active infection, as PCR detects the presence of the virus, and not the hosts reaction to the virus.  Polyomavirus BK DNA detection in urine or plasma is associated with an increased risk of graft rejection in renal recipients.  Quantitative testing may indicate change in risk over time.  This is a quantitative molecular test, with a linear range of 500-500,000,000 copies/mL.
Custom Panel: No


Turn Around Time: 1 day
Days Performed: Sunday, Monday, Tuesday, Wednesday, Thursday, Friday, Saturday
Sites Performed: Quest - Chantilly
PHL Test Code: BKSE3
EPIC Test Code: LAB3240
Send Out Test Code: 11274
Alternate Test Names: BK Virus DNA, Quant PCR, BKV Polyoma, BK Virus DNA; BK Virus, Serum; BK Virus, Serum Quantitative ; BKV; BKV, Serum
Included Tests:  
CPT Coding: 87799

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