Medical Necessity Documentation:
Client Notes:
Patient Preparation:
Specimen Requirements:
5 mg small bowel biopsy collected in a 2 mL cryovial polypropylene screw-capped tube
Collection Instructions:
The tissue should not be placed on gauze or filter paper, nor should any saline, support or embedding material be added
Place 5 mg (2 mg minimum) small bowel biopsy on the interior surface of a 2 mL polypropylene screw-capped cryovial or small polypropylene collection tube.
Freeze within 2 hours of collection
Minimum Volume:
2 mg tissue
Transport & Storage: Temperature/Stability:
Unacceptable Ambient
Unacceptable Refrigerated
31 days Frozen
Rejection Criteria:
Samples frozen later than 2 hours post-collection • Samples received in urine containers • Samples immersed in a colorless fluid (i.e., water, formalin, saline, etc.) • Samples placed on gauze, filter paper or any other type of support media
Reference Range:
| Lactase |
15.0-45.5 uM/min/g prot |
| Sucrase |
25.0-69.9 uM/min/g prot |
| Maltase |
100.0-224.4 uM/min/g prot |
| Palatinase |
05-26.3 uM/min/g prot |
Critical Ranges:
Test Comments:
Methodology:
Spectrophotometry (SP)
Clinical Significance:
Lactase, maltase, palatinase and sucrase are mucosal disaccharidases that are present in normal small intestine and fetal colon. A decrease in the enzyme activity of these disaccharidases has been noted in colonic adenomas, adenocarcinomas and malabsorption. Decreased disaccharidase activity is a good indication of mucosal injury with the exception of lactase
Documentation:
Custom Panel:
No
PRODUCTION SCHEDULE
Turn Around Time:
2 to 4 days
Days Performed:
Sunday, Monday, Tuesday, Wednesday, Thursday
Sites Performed:
Quest - Chantilly
PHL Test Code:
MSOT
EPIC Test Code:
MISC
Send Out Test Code:
19701
Alternate Test Names:
Disaccharidase Analysis; Disaccharidase Assay; Disaccharidase Assay, Tissue; Disaccharidase Determination; Disaccharide
Included Tests:
Lactase level, Maltase level, Palatinase level, Sucrase level
CPT Coding:
82657 x 4