Disaccharidase

Included Tests

Lactase level, Maltase level, Palatinase level, Sucrase level

Clinical Significance

Lactase, maltase, palatinase and sucrase are mucosal disaccharidases that are present in normal small intestine and fetal colon. A decrease in the enzyme activity of these disaccharidases has been noted in colonic adenomas, adenocarcinomas and malabsorption. Decreased disaccharidase activity is a good indication of mucosal injury with the exception of lactase

Specimen Requirements

5 mg small bowel biopsy collected in a 2 mL cryovial polypropylene screw-capped tube

Alternate Specimen

TransportAndStorage

Unacceptable Ambient
Unacceptable Refrigerated
31 days Frozen

Collection Instructions

The tissue should not be placed on gauze or filter paper, nor should any saline, support or embedding material be added

Place 5 mg (2 mg minimum) small bowel biopsy on the interior surface of a 2 mL polypropylene screw-capped cryovial or small polypropylene collection tube.


Freeze within 2 hours of collection

Minimum Volume

2 mg tissue

Neonatal Volumne

Reference Range:

Lactase	15.0-45.5 uM/min/g prot
Sucrase	25.0-69.9 uM/min/g prot
Maltase	100.0-224.4 uM/min/g prot
Palatinase	05-26.3 uM/min/g prot

Methodology:

• Spectrophotometry (SP)

Clinical Significance

Lactase, maltase, palatinase and sucrase are mucosal disaccharidases that are present in normal small intestine and fetal colon. A decrease in the enzyme activity of these disaccharidases has been noted in colonic adenomas, adenocarcinomas and malabsorption. Decreased disaccharidase activity is a good indication of mucosal injury with the exception of lactase

CDM

00917200

CPT Coding

82657 x 4