BK Virus DNA, Quantitative, Urine

Overview

  • EPIC Code:
  • LAB3241
  • Soft Test Code:
  • BKUR3
  • Send Out Test Code:
  • 16581
Alternate Names
  • BK Virus DNA, Quant PCR, Urine, BKV Polyoma, BK Vi
  • BK Virus DNA, Quantitative Real-Time PCR, Urine
  • BK Virus, Urine
  • BK Virus, Urine Quantitative
  • BKV
  • BKV, Urine
Clinical Significance

This test detects and quantitates BK Virus DNA in urine specimens of kidney transplant recipients who are at risk of developing BKV-associated nephropathy (BKVAN) and associated hemorrhagic cystitis leading to kidney failure. Prospective viral load monitoring of BKV DNA in urine (and plasma) is useful to exclude a diagnosis of BKVAN and to monitor the course of BKV infection. The assay is calibrated to the First World Health Organization International Standard for BKV DNA with a Reportable Range of 200-100,000,000 IU/mL (2.30-8.00 log IU/mL).


Specimen Collection & Preparation

Specimen Requirements:

-OR-

Urine in a Yellow Top Tube - PCR Media
Minimum Volume:
4.3 mL*

* This volume does not allow for repeat testing

Specimen Information:

Collect

Pipette into


Collection Instructions:

Unpreserved urine must be immediately stabilized by transferring to a PCR Media tube.  Liquid level must be between the black fill lines on the cobas® PCR Media tube label window.


Processing Instructions:

  1. Immediately pipette urine into the cobas® PCR Media tube using the provided disposable pipette.
    1. If not enough volume of urine is collected (less than 4.3 mL) send urine in the collection cup to laboratory.
  2. Pipette the correct volume of urine, this is met when the fluid level is between the two black lines on the tube label.
  3. Tightly re-cap the cobas® PCR Media tube.
  4. Invert the tube 5 times to mix. 


Rejection Criteria:

Under-or over-filled cobas® PCR urine sample tubes

Unpreserved urine

Frozen urine


Transport and Storage:
  • Ambient (18-24°C): 90 days
  • Refrigerated (2-8°C): 90 days

    If the urine specimen cannot be transferred immediately to the cobas® PCR Media tube, it can be stored at 2˚C - 30˚C for up to 24 hours.

    **Transport refrigerated.

Clinical Interpretation

Reference Range:

BK Virus DNA, QN PCR Not Detected (IU/mL)
BK Virus DNA, QN PCR Not Detected (Log IU/mL)


Methodology:
  • Real-Time Polymerase Chain Reaction

Clinical Significance

This test detects and quantitates BK Virus DNA in urine specimens of kidney transplant recipients who are at risk of developing BKV-associated nephropathy (BKVAN) and associated hemorrhagic cystitis leading to kidney failure. Prospective viral load monitoring of BKV DNA in urine (and plasma) is useful to exclude a diagnosis of BKVAN and to monitor the course of BKV infection. The assay is calibrated to the First World Health Organization International Standard for BKV DNA with a Reportable Range of 200-100,000,000 IU/mL (2.30-8.00 log IU/mL).


Production Schedule

Sites Performed
  • Quest - Chantilly
Days Performed
Sunday
Monday
Tuesday
Wednesday
Thursday
Friday
Saturday
Departments
  • Sendouts - Clinical
Turn Around Time

1 day


Coding & Compliance

CPT Coding

87799